Spina C A, Dorey F, Vescera C, Brosman S, Fahey J L
Cancer Res. 1981 Nov;41(11 Pt 1):4324-30.
Depressed T-lymphocyte function as assessed by delayed-type hypersensitivity reactions and in vitro proliferative response to mitogens is a characteristic finding in many types of solid tumors, including bladder carcinoma. Peripheral blood leukocytes from 16 patients with transitional cell carcinoma of the bladder were compared with age-matched, control subjects. Both the unfractionated leukocytes containing 10 to 30% monocytes and the lymphocyte-enriched preparations, obtained by monocyte depletion with iron filing ingestion, were analyzed. Mixed leukocyte culture-induced cytotoxicity was depressed in the patient group; the amount of depression was directly correlated to the extent of the disease. In patients who underwent surgical removal of tumor, the mixed leukocyte culture-induced cytotoxicity appeared normal. This mixed leukocyte culture-generated cytotoxic response was a more sensitive indicator of tumor effect than was the induced proliferative response. Removal of phagocytic or adherent monocytes from the responding cell population caused a significant increase in the generated cytotoxicity, especially in those patients with invasive disease. These suppressive effects could be partially reconstituted by quantitative addition of the separated monocytes back to the responding lymphocyte culture. The depressed lymphocyte-mediated cytotoxicity present in these bladder cancer patients was due, in a major part, to a circulating macrophage-like cell with active suppressor function.
通过迟发型超敏反应和对有丝分裂原的体外增殖反应评估的T淋巴细胞功能低下是包括膀胱癌在内的多种实体瘤的一个特征性发现。将16例膀胱移行细胞癌患者的外周血白细胞与年龄匹配的对照受试者进行比较。对含有10%至30%单核细胞的未分离白细胞以及通过铁屑吞噬去除单核细胞获得的富含淋巴细胞的制剂进行了分析。患者组中混合白细胞培养诱导的细胞毒性降低；降低程度与疾病程度直接相关。在接受肿瘤手术切除的患者中，混合白细胞培养诱导的细胞毒性似乎正常。这种混合白细胞培养产生的细胞毒性反应比诱导的增殖反应更能敏感地反映肿瘤效应。从反应细胞群体中去除吞噬性或黏附性单核细胞会导致产生的细胞毒性显著增加，尤其是在那些患有侵袭性疾病的患者中。通过将分离的单核细胞定量添加回反应性淋巴细胞培养物中，这些抑制作用可以部分恢复。这些膀胱癌患者中存在的淋巴细胞介导的细胞毒性降低主要是由于具有活性抑制功能的循环巨噬细胞样细胞所致。
