Behavioral, ovarian and endocrine relationships in the pubertal bitch.

Behavioral, ovarian and endocrine correlates were determined in seven bitches experiencing first estrus, and the results compared with previous data obtained on multiparous females. One or more atypical reproductive patterns were observed in certain bitches at the pubertal estrus. The ability of pubertal dogs to display normal reproductive relationships appeared to be related to age, because animals that exhibited normal sexual behavior and endocrine profiles tended to be older than females that produced aberrant patterns. For the latter group, atypical observations included lack of sexual receptivity and reduced or inconsistent patterns of circulating concentrations of estradiol-17 beta, luteinizing hormone (LH) or progesterone. Ovulation was confirmed in all four pubertal dogs subjected to laparoscopy, including two bitches that failed to demonstrate estrous behavior. In individual animals, it appeared that inadequate or sporadic elevations in titers of estradiol-17 beta during the preovulatory phase of the cycle were associated with reduced or abbreviated LH surge concentrations, and the latter were subsequently related to an absent or only minor preovulatory rise in serum progesterone. Reduced preovulatory estradiol-17 beta concentrations and a delayed increase in circulating progesterone were also associated with a lack of sexual receptivity. These results (1) suggest that hormonal insufficiencies or irregularities exist in certain bitches at the pubertal estrus and that such atypical activity appears more characteristic of the first than later adult estrous periods, and (2) are consistent with the theory that estrogen preconditioning and a decrease in the endogenous estrogen to progesterone ratio are important in the initiation of sexual receptivity in this species.