Osada H, Ward C A, Duffin J, Nelems J M, Cooper J D
Am J Physiol. 1978 May;234(5):H646-52. doi: 10.1152/ajpheart.1978.234.5.H646.
We tested the hypothesis that platelet loss following blood contact with foreign materials is partly related to the presence of microbubbles of gas (gas nuclei) trapped in surface defects on the membrane material. Extracorporeal membrane oxygenator perfusions were conducted in two groups of sheep, with use of standard priming techniques for the oxygenator in one group and a new vacuum priming technique in the other group. The vacuum priming technique was developed to eliminate gas nuclei from the oxygenator surface. With denucleation priming, platelet loss during perfusion was markedly reduced, as was thrombus formation on the membrane surface. The platelet particle-size distribution curve showed a shift consistent with platelet aggregation with the standard priming technique but not with the vacuum priming technique. We conclude that the elimination of trapped gas nuclei from the membrane surface during priming reduces the initial interaction between blood elements and the foreign surface.
血液与异物接触后血小板损失部分与被困在膜材料表面缺陷中的气体微泡（气核）的存在有关。对两组绵羊进行体外膜肺氧合灌注，一组使用氧合器的标准预充技术，另一组使用新的真空预充技术。开发真空预充技术是为了消除氧合器表面的气核。采用去核预充时，灌注过程中的血小板损失显著减少，膜表面的血栓形成也减少。血小板粒径分布曲线显示，标准预充技术会出现与血小板聚集一致的偏移，而真空预充技术则不会。我们得出结论，预充过程中从膜表面消除被困气核可减少血液成分与异物表面之间的初始相互作用。
