Takaoki M, Sy M S, Whitaker B, Nepom J, Finberg R, Germain R N, Nisonoff A, Benacerraf B, Greene M I
J Immunol. 1982 Jan;128(1):49-53.
Biologic activities and immunochemical characteristics of an azobenzenearsonate- (ABA)specific suppressor T cell factor produced by a longterm T cell hybridoma, F12, were studied. In vivo administration of F12 culture supernatant resulted in the suppression of ABA-specific delayed-type hypersensitivity (DTH) responses and the inhibition of priming for ABA-specific cytotoxic T lymphocyte responses. Moreover, F12 induced a second set of suppressor cells that act in the efferent phase of DTH. The active material in the F12 culture supernatant expressed major cross-reactive idiotypic (CRI) determinants of anti-ABA antibodies of A/J mice and I-J subregion-coded specificities but not express determinants of immunoglobulin constant regions. These results demonstrated that F12 is a functioning hybrid cell line of the first-order suppressor T cell subset.
研究了长期T细胞杂交瘤F12产生的偶氮苯砷酸盐特异性抑制性T细胞因子的生物学活性和免疫化学特性。体内给予F12培养上清液可抑制ABA特异性迟发型超敏反应（DTH），并抑制ABA特异性细胞毒性T淋巴细胞反应的致敏。此外，F12诱导了一组在DTH传出阶段起作用的抑制性细胞。F12培养上清液中的活性物质表达了A/J小鼠抗ABA抗体的主要交叉反应独特型（CRI）决定簇和I-J亚区域编码的特异性，但不表达免疫球蛋白恒定区的决定簇。这些结果表明F12是一级抑制性T细胞亚群的功能性杂交细胞系。
