[Nicotinamide-adenine dinucleotide synthesis by microorganisms].

The ability of five bacterial strains, i.e., Brevibacterium ammoniagenes ATCC 6872, Brevibacterium flavum ATCC 14067, Brevibacterium 22, Corynebacterium ATCC 21084, Micrococcus glutamicus ATCC 13032, to utilize exogenous precursors (nicotinamide and adenine or ATP) was investigated during NAD synthesis under fermentation conditions and during incubation of acetone-dried cells. It was found that dry cells of Brevibacterium three strains were most active. However, under fermentation conditions Br. flavum ATCC 14067 and Brevibacterium 22 accumulated NAD in the amounts 3J4 times lower than the well-known producer Br. ammoniagenes ATCC 6872. One of the possible factors responsible for the low yield of NAD by Brevibacterium 22 under fermentation conditions can be the reduced ribose synthesis.