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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Fähnrich M, Ahlers J

Eur J Biochem. 1981 Dec;121(1):113-8. doi: 10.1111/j.1432-1033.1981.tb06438.x.

An improved filtration method is introduced to perform kinetic investigations on the chitin synthase reaction. This method is especially suited for the assay of a large number of samples necessary in enzyme kinetic studies. 2. From initial rate data the possibility could be excluded that the two-substrate reactions occurs by a random or a ping-pong mechanism. 3. Investigations of product inhibition exclude a rapid random mechanism but favour an ordered mechanism with UDP-N-acetylglucosamine as the first substrate. 4. This result was confirmed by isotope exchange studies.

引入了一种改进的过滤方法来对几丁质合酶反应进行动力学研究。该方法特别适用于酶动力学研究中所需的大量样品的测定。2. 根据初始速率数据，可以排除双底物反应通过随机或乒乓机制发生的可能性。3. 产物抑制研究排除了快速随机机制，但支持以UDP-N-乙酰葡糖胺作为第一个底物的有序机制。4. 同位素交换研究证实了这一结果。

Fähnrich M, Ahlers J

Eur J Biochem. 1981 Dec;121(1):113-8. doi: 10.1111/j.1432-1033.1981.tb06438.x.

An improved filtration method is introduced to perform kinetic investigations on the chitin synthase reaction. This method is especially suited for the assay of a large number of samples necessary in enzyme kinetic studies. 2. From initial rate data the possibility could be excluded that the two-substrate reactions occurs by a random or a ping-pong mechanism. 3. Investigations of product inhibition exclude a rapid random mechanism but favour an ordered mechanism with UDP-N-acetylglucosamine as the first substrate. 4. This result was confirmed by isotope exchange studies.

引入了一种改进的过滤方法来对几丁质合酶反应进行动力学研究。该方法特别适用于酶动力学研究中所需的大量样品的测定。2. 根据初始速率数据，可以排除双底物反应通过随机或乒乓机制发生的可能性。3. 产物抑制研究排除了快速随机机制，但支持以UDP-N-乙酰葡糖胺作为第一个底物的有序机制。4. 同位素交换研究证实了这一结果。