Ptak W, Green D R, Durum S K, Kimura A, Murphy D B, Gershon R K
Eur J Immunol. 1981 Dec;11(12):980-3. doi: 10.1002/eji.1830111206.
The intravenous injection of 2,4,6-trinitrophenyl (TNP)-labeled peritoneal exudate cells (TNP-PEC) into CBA mice fails to produce a state of hypersensitivity; rather, it renders recipient mice incapable of mounting a contact hypersensitivity response when they are subsequently immunized with a reactive form of the specific hapten. However, if precultured neonatal spleen cells are injected along with the cells that induce tolerance (TNP-PEC), not only is the development of tolerance inhibited but sensitization to TNP develops. The neonatal spleen cell responsible for turning the tolerogenic signal into an immunogenic one is I-J+ and adheres to the Vicia villosa lectin. Thus, it expresses markers that distinguish contrasuppressor effector cells from helper cells (D. R. Green et al., Eur. J. Immunol. 1981. 11:973), indicating that activated contrasuppressor cells can act as potent, helpful regulatory cells in vivo.
将2,4,6-三硝基苯基（TNP）标记的腹腔渗出细胞（TNP-PEC）静脉注射到CBA小鼠体内，不会产生超敏反应状态；相反，当随后用特异性半抗原的反应形式免疫时，受体小鼠无法产生接触性超敏反应。然而，如果将预培养的新生脾细胞与诱导耐受的细胞（TNP-PEC）一起注射，不仅耐受的发展受到抑制，而且对TNP产生了致敏作用。负责将致耐受信号转变为免疫原性信号的新生脾细胞是I-J+，并能黏附于绒毛野豌豆凝集素。因此，它表达的标志物可将抗抑制效应细胞与辅助细胞区分开来（D.R.格林等人，《欧洲免疫学杂志》，1981年。11:973），这表明活化的抗抑制细胞在体内可作为强大的辅助调节细胞发挥作用。
