Robbins F M, Hartzman R J
Hum Immunol. 1981 Oct;3(2):121-31. doi: 10.1016/0198-8859(81)90049-5.
Human lymphocytes, 10 days after treatment with phytohemagglutinin P (PHA-P), show PLT (secondary in vitro restimulation) reaction patterns which correspond to their cellular (HLA-D) type. Lymphocytes with shared cellular type stimulate PHA primed cells to a lesser degree than the majority of lymphocytes with unshared HLA-D type, which stimulate a strong response. Using a standard 3H thymidine incorporation technique, it is possible to detect responses in as early as 12 hr total incubation time, although usually a 22-48 hr total incubation time is normally required. This method is simple, gives results that correspond to the primary mixed leukocyte response, and thus may be useful as a cross-matching technique for cadaveric renal transplantation.
用植物血凝素P（PHA-P）处理10天后的人淋巴细胞表现出与它们的细胞（HLA-D）类型相对应的PLT（二次体外再刺激）反应模式。具有相同细胞类型的淋巴细胞比大多数具有不同HLA-D类型的淋巴细胞对PHA预致敏细胞的刺激程度要小，后者能刺激强烈反应。使用标准的3H胸苷掺入技术，即使通常总共需要22 - 48小时的孵育时间，但在总共仅12小时的孵育时间内就有可能检测到反应。该方法简单，结果与初次混合淋巴细胞反应相对应，因此可用作尸体肾移植的交叉配型技术。
