表皮朗格汉斯细胞对一种用于显示β-葡萄糖醛酸酶的组织化学方法的反应性。

Reactivity of epidermal Langerhans cells to a histochemical method for demonstration of beta-glucuronidase.

作者信息

Mackenzie I C, Bickenbach J R, Rittman B R

出版信息

J Invest Dermatol. 1982 Mar;78(3):239-42. doi: 10.1111/1523-1747.ep12506562.

Abstract

Sheets or sections of mouse epidermis reacted by a histochemical method for the enzyme beta-glucuronidase display a subpopulation of dendritic cells which correspond in number and spacing to Langerhans cells demonstrated by reactivity for ATPase or Ia antigens. A similar staining pattern is seen in rat, rabbit, and guinea pig epidermis. In rhesus monkey and human skin, Langerhans cells appear to be reactive for beta-glucuronidase but, as keratinocytes are also reactive, Langerhans cells are not readily identifiable by this method. The thermal stability of beta-glucuronidase differs between strains of mice. Langerhans cells of Balb/C and C3H strains can thus be distinguished by appropriate pretreatment before incubation, a method of potential value for experimental investigations of the origin of Langerhans cells.

摘要

用组织化学方法对小鼠表皮切片或部分进行β-葡萄糖醛酸酶反应，显示出一群树突状细胞，其数量和间距与通过ATP酶或Ia抗原反应所证实的朗格汉斯细胞相对应。在大鼠、兔子和豚鼠的表皮中也观察到类似的染色模式。在恒河猴和人类皮肤中，朗格汉斯细胞似乎对β-葡萄糖醛酸酶有反应，但由于角质形成细胞也有反应，用这种方法不容易识别朗格汉斯细胞。不同品系小鼠的β-葡萄糖醛酸酶热稳定性不同。因此，通过孵育前的适当预处理，可以区分Balb/C和C3H品系的朗格汉斯细胞，这是一种对朗格汉斯细胞起源进行实验研究具有潜在价值的方法。