Tilly K, McKittrick N, Georgopoulos C, Murialdo H
Prog Clin Biol Res. 1981;64:35-45.
We have previously reported the isolation of E. coli groE mutants, which block lambda head morphogenesis. Further analysis of these mutants showed that many are temperature-sensitive for bacterial growth or block the growth of the unrelated phages T4 and T5. We have established the existence of a second groE gene, which we call groES, that is different from the previously described groE gene encoding a 65,000-Mr polypeptide (Georgopoulos and Hohn, 1978; Hendrix and Tsui, 1978) and which is renamed groEL. Genetic and biochemical studies of lambda groE+ transducing phages and their mutant derivatives show that these two genes are closely linked and that the groES gene codes for a polypeptide of 15,000-Mr. Bacterial groEL- or groES- mutants exhibit the same growth kinetics and phenotype at high temperature and lambda proheads have the same protein composition in both classes of mutants.
我们之前报道了大肠杆菌groE突变体的分离，这些突变体阻断了λ噬菌体头部形态发生。对这些突变体的进一步分析表明，许多突变体对细菌生长是温度敏感的，或者阻断无关噬菌体T4和T5的生长。我们确定了第二个groE基因的存在，我们将其称为groES，它与先前描述的编码65,000道尔顿多肽的groE基因不同（乔治opoulos和霍恩，1978年；亨德里克斯和崔，1978年），后者现重新命名为groEL。对λ groE+转导噬菌体及其突变衍生物的遗传和生化研究表明，这两个基因紧密连锁，且groES基因编码一种15,000道尔顿的多肽。细菌groEL-或groES-突变体在高温下表现出相同的生长动力学和表型，并且在这两类突变体中λ原头部具有相同的蛋白质组成。
