Tilly K, Georgopoulos C
J Bacteriol. 1982 Mar;149(3):1082-8. doi: 10.1128/jb.149.3.1082-1088.1982.
The Escherichia coli groEL and groES gene products are essential for both phage morphogenesis and bacterial growth. Although the gene products have been identified, their exact roles in these processes are not known. We have isolated mutations in the groEL gene that suppress defects in the groES gene. These intergenic suppressors were shown to map in the groEL gene by a variety of genetic and biochemical analyses. These results suggest that the two morphogenetic gene products interact in vivo and help to explain why mutations in either gene exhibit the same phenotype with respect to lambda head assembly and bacterial growth.
大肠杆菌的groEL和groES基因产物对于噬菌体形态发生和细菌生长都是必不可少的。尽管已经鉴定出了这些基因产物，但它们在这些过程中的确切作用尚不清楚。我们分离出了groEL基因中的突变，这些突变能够抑制groES基因中的缺陷。通过各种遗传和生化分析表明，这些基因间抑制子定位于groEL基因中。这些结果表明，这两种形态发生基因产物在体内相互作用，这有助于解释为什么任一基因中的突变在λ噬菌体头部组装和细菌生长方面表现出相同的表型。
