纤溶酶处理的人免疫球蛋白制剂的抗体谱及抗补体活性

Antibody spectrum and anticomplementary activity of a plasmin-treated human immunoglobulin preparation.

作者信息

Masuda K, Makula M F, Okuyama K, Ishiyama H, Yasuda J

出版信息

Vox Sang. 1982 Jan;42(1):8-15. doi: 10.1111/j.1423-0410.1982.tb01064.x.

DOI:10.1111/j.1423-0410.1982.tb01064.x

Abstract

A commercial plasmin-treated human immunoglobulin was studied for its antibody spectrum and anticomplementary activity. Except for a decrease in anti-vaccinia antibody titers in two of three paired samples collected before and after plasmin treatment, there was practically no fall in titers of diphtheria antitoxin and of antibodies to measles, rubella and mumps viruses. Among the three major fractions isolated from the preparation, the plasmin-resistant 7S IgG fraction showed the highest anticomplementary activity when tested by our routine method described in the "Japanese Minimum Requirements'. The Fab fraction showed moderate anticomplementary activity which was thought to be due to the selective consumption of C3. On the contrary, the Fc fraction was not found to be anticomplementary in our test method. In some of the above-mentioned features, the preparation seems to be different from the domestic product formerly studied by ourselves.

摘要

对一种经商业纤溶酶处理的人免疫球蛋白的抗体谱和抗补体活性进行了研究。除了在纤溶酶处理前后采集的三对样本中有两对的抗牛痘抗体效价有所下降外，白喉抗毒素以及针对麻疹、风疹和腮腺炎病毒的抗体效价实际上并未降低。从该制剂中分离出的三个主要组分中，采用我们在《日本最低要求》中描述的常规方法进行检测时，抗纤溶酶的7S IgG组分显示出最高的抗补体活性。Fab组分显示出中等程度的抗补体活性，这被认为是由于C3的选择性消耗所致。相反，在我们的检测方法中未发现Fc组分具有抗补体活性。在上述一些特征方面，该制剂似乎与我们之前研究过的国产产品有所不同。