Goeken N E, Thompson J S
Hum Immunol. 1982 Feb;4(1):37-45. doi: 10.1016/0198-8859(82)90048-9.
Theophylline, a phosphodiesterase inhibitor, also reversibly inhibits the SRBC receptor of a subpopulation of human T lymphocytes. We have previously reported that the precursor for the Concanavalin A (Con A) inducible suppressor cell (SC) is found predominantly in the theophylline-sensitive (Ts) population; whereas, the precursor of the mitomycin resistant suppressor cell induced in the allogeneic mixed lymphocyte reaction (MLR) is predominantly in the theophylline-resistant (TR) subset. This study examines the theophylline sensitivity and functional characteristics of suppressor cells induced in autologous MLR (AMLR). Both TS and TR proliferate moderately in AMLR but differ in their ability to form suppressors. AMLR activated Ts and TR cells demonstrate early blastogenesis and weak suppression in the Con A assay system, but suppression in an MLR assay system appeared to be confined largely to the TR subset. AMLR suppressors were mitomycin sensitive. These data illustrate that suppressors generated in allogeneic and autologous MLR have significant functional and phenotypic similarities, and strengthen the association between these two immunologic phenomena.
茶碱是一种磷酸二酯酶抑制剂，它还能可逆性抑制人T淋巴细胞亚群的SRBC受体。我们之前报道过，伴刀豆球蛋白A（Con A）诱导的抑制性细胞（SC）的前体主要存在于对茶碱敏感（Ts）的群体中；而在同种异体混合淋巴细胞反应（MLR）中诱导产生的丝裂霉素抗性抑制性细胞的前体主要存在于对茶碱耐药（TR）的亚群中。本研究检测了自体MLR（AMLR）中诱导产生的抑制性细胞对茶碱的敏感性及其功能特性。Ts和TR在AMLR中均有适度增殖，但在形成抑制性细胞的能力方面存在差异。AMLR激活的Ts和TR细胞在Con A检测系统中表现出早期母细胞形成和微弱的抑制作用，但在MLR检测系统中的抑制作用似乎主要局限于TR亚群。AMLR抑制性细胞对丝裂霉素敏感。这些数据表明，同种异体和自体MLR中产生的抑制性细胞在功能和表型上有显著相似性，并加强了这两种免疫现象之间的关联。
