Fuller L, Flaa C, Jaffe D, Strauss J, Kyriakides G K, Miller J
J Clin Invest. 1983 May;71(5):1322-30. doi: 10.1172/jci110883.
In long-term well adapted kidney transplant recipients we have found a close correlation between the T helper (TH):T suppressor/cytotoxic (TS/C) subset ratios and the presence of T cells that respond in the autologous mixed lymphocyte reaction (AMLR). In 21 recipients with T cell E rosette levels ranging between 53 and 86% and TH:TS/C ratios between 0.15 to 2.10, ratios of greater than 0.8 correlated with AMLR responses (13/13), and ratios of less than 0.8 with AMLR nonreactivity (7/7). By contrast, the allogeneic MLR showed no apparent correlation with the TH:TS/C ratios or with the AMLR pre- or postoperatively. It was found that the AMLR in 22 of 23 normal individuals was markedly inhibited by autologous T cells obtained from peripheral blood lymphocytes, exposed to 3,000 rad (Tx) and added as a third component to the cultures. In contrast, 13 of 13 kidney transplant recipients failed to exhibit this Tx AMLR inhibitory cell population. The "naturally occurring" T inhibitory cells, fractionated by an affinity column chromatography procedure into x-irradiated TH and TS/C subsets, inhibited the AMLR to the same extent as unseparated Tx cells. In cell interchange studies performed in four of five HLA identical donor-recipient pairs the Tx cells of the (normal) donor inhibited the recipient AMLR (immunosuppressed), but recipient Tx cells failed to inhibit the donor AMLR. Finally T cells, primed in AMLR and allogeneic MLR for 10 d were tested for AMLR or allogeneic MLR inhibitory activity. Allogeneic MLR primed x-irradiated cells, inhibited both the AMLR and allogeneic MLR while AMLR x-irradiated primed cells inhibited neither reaction. The Tx AMLR inhibitor found in normal peripheral blood, appears to be a cell that is highly sensitive to the effects of biologic or pharmacologic immunosuppressive agents.
在长期适应性良好的肾移植受者中，我们发现辅助性T细胞（TH）与抑制性/细胞毒性T细胞（TS/C）亚群比例和在自体混合淋巴细胞反应（AMLR）中有反应的T细胞的存在之间存在密切关联。在21名T细胞E花环水平在53%至86%之间且TH:TS/C比例在0.15至2.10之间的受者中，大于0.8的比例与AMLR反应相关（13/13），小于0.8的比例与AMLR无反应相关（7/7）。相比之下，同种异体混合淋巴细胞反应（MLR）与TH:TS/C比例或术前术后的AMLR均无明显相关性。研究发现，23名正常个体中有22名的AMLR受到从外周血淋巴细胞获取的自体T细胞的显著抑制，这些T细胞经3000拉德（Tx）照射后作为第三成分添加到培养物中。相反，13名肾移植受者均未表现出这种Tx AMLR抑制细胞群。通过亲和柱层析程序分离成经x射线照射的TH和TS/C亚群的“天然存在”的T抑制细胞，对AMLR的抑制程度与未分离的Tx细胞相同。在五对HLA相同的供者-受者对中的四对进行的细胞交换研究中，（正常）供者的Tx细胞抑制了受者的AMLR（免疫抑制），但受者的Tx细胞未能抑制供者的AMLR。最后，对在AMLR和同种异体MLR中预刺激10天的T细胞进行AMLR或同种异体MLR抑制活性测试。同种异体MLR预刺激的经x射线照射的细胞抑制了AMLR和同种异体MLR，而AMLR预刺激的经x射线照射的细胞对两种反应均无抑制作用。在正常外周血中发现的Tx AMLR抑制剂似乎是一种对生物或药物免疫抑制剂的作用高度敏感的细胞。
