Hanson D G, Miller S D
J Immunol. 1982 May;128(5):2378-81.
The effect of CY pretreatment on the ability of OVA feeding to induce both tolerance and active suppression was examined in mice. CY-pretreated, OVA fed mice were fully unresponsive in both OVA-specific DTH and antibody responses, but, in contrast to untreated OVA-fed mice, did not transfer suppression to normal recipients via splenic lymphocytes. Restoration of Ts activity in CY-pretreated mice was accomplished by reconstitution with normal T cells before antigen feeding, indicating that the CY effect was at the Ts precursor level. In addition, it was found that certain OVA-specific immune parameters (DTH and splenic PFC responses) in recipient mice were susceptible to suppression by transfer of spleen cells from OVA-fed donors, whereas other measures (antigen-induced T cell proliferation and serum antibody titers) were not. The data suggest that CY-sensitive Ts are not necessary for either induction or maintenance of specific tolerance after OVA feeding.
在小鼠中检测了环磷酰胺（CY）预处理对卵清蛋白（OVA）喂养诱导耐受和主动抑制能力的影响。经CY预处理且喂食OVA的小鼠在OVA特异性迟发型超敏反应（DTH）和抗体反应中均完全无反应，但与未处理的喂食OVA的小鼠不同，它们不能通过脾淋巴细胞将抑制作用传递给正常受体。通过在抗原喂养前用正常T细胞重建，恢复了经CY预处理小鼠的Ts活性，这表明CY的作用是在Ts前体水平。此外，还发现受体小鼠中的某些OVA特异性免疫参数（DTH和脾空斑形成细胞反应）易受来自喂食OVA供体的脾细胞转移的抑制，而其他指标（抗原诱导的T细胞增殖和血清抗体滴度）则不受影响。数据表明，CY敏感的Ts对于OVA喂养后特异性耐受的诱导或维持并非必需。
