Lamont A G, Gordon M, Ferguson A
Immunology. 1987 Jul;61(3):339-43.
The induction of oral tolerance for DTH responses to ovalbumin is impaired in protein-deprived mice. This may be via the effects of protein malnutrition on short-lived Ts cells, but an alternative explanation is that the gut handling of antigen is abnormal. We have attempted to transfer tolerance from protein-deprived and control mice to naive recipients by using spleen cells, collected 7 days after an OVA feed at a time when Ts cells should be present, or by using serum, collected 1 hr after feeding, which should contain tolerogenic, 'gut-processed' antigen. Suppression of DTH was transferred with 7-day spleen cells and with 1-hr serum from normal, protein-sufficient mice. Mice that received spleen cells from protein-deprived donors were not tolerant, but suppression was readily transferred with serum from deprived mice, indicating that their capacity for intestinal antigen processing was normal. Furthermore, the quantity of absorbed antigen in the serum 1 hr after feeding was similar in both protein-deprived and normal groups. The results obtained are consistent with the hypothesis that short-term protein deprivation depletes a population of short-lived Ts cells which control DTH oral tolerance.
蛋白质缺乏的小鼠对卵清蛋白迟发型超敏反应的口服耐受诱导受损。这可能是由于蛋白质营养不良对短命 Ts 细胞的影响，但另一种解释是肠道对抗原的处理异常。我们试图通过使用在给予卵清蛋白 7 天后收集的脾细胞（此时 Ts 细胞应该存在），或者通过使用喂食 1 小时后收集的血清（其中应该含有致耐受性的“肠道处理过的”抗原），将蛋白质缺乏小鼠和对照小鼠的耐受性转移给未接触过抗原的受体。正常、蛋白质充足小鼠的 7 天脾细胞和 1 小时血清可转移迟发型超敏反应的抑制作用。接受蛋白质缺乏供体脾细胞的小鼠不耐受，但用缺乏蛋白质小鼠的血清很容易转移抑制作用，这表明它们肠道抗原处理能力正常。此外，喂食 1 小时后血清中吸收的抗原量在蛋白质缺乏组和正常组中相似。所获得的结果与以下假设一致，即短期蛋白质缺乏会耗尽控制迟发型超敏反应口服耐受的短命 Ts 细胞群体。
