Paz G F, Winter J S, Reyes F I, Faiman C
Int J Androl. 1982 Feb;5(1):74-80. doi: 10.1111/j.1365-2605.1982.tb00235.x.
A method for the determination of delta 5 3 beta-hydroxysteroid dehydrogenase-isomerase (3 beta-HSD) activity in intact isolated Leydig cells was established. This method utilizes the conversion of [7-3H]dehydroepiandrosterone (1.04 mumole) to androstenedione and expresses the activity of the enzyme as mumoles of androstenedione produced/microgram DNA/h. The reaction is limited to 0.5 - 4 micrograms DNA of Leydig cells/ml (equivalent to 0.1-0.8 million of Leydig cells/ml) and to 1 h of incubation at 34 degree C. The 3 beta-HSD activity of 44 suspensions of Leydig cells isolated from adult rats was found to be 1.13 +/- 0.03 (SE) mumoles/microgram DNA/h. This new method for direct measurement of 3 beta-HSD activity in intact Leydig cells was found to be rapid, easy to perform and highly reproducible.
建立了一种测定完整分离的睾丸间质细胞中δ5 3β-羟基类固醇脱氢酶-异构酶（3β-HSD）活性的方法。该方法利用[7-3H]脱氢表雄酮（1.04微摩尔）转化为雄烯二酮，并将酶的活性表示为每微克DNA每小时产生的雄烯二酮微摩尔数。反应限于每毫升睾丸间质细胞0.5 - 4微克DNA（相当于每毫升0.1 - 0.8百万个睾丸间质细胞），并在34℃孵育1小时。从成年大鼠分离的44个睾丸间质细胞悬液的3β-HSD活性为1.13±0.03（SE）微摩尔/微克DNA/小时。发现这种直接测量完整睾丸间质细胞中3β-HSD活性的新方法快速、易于操作且高度可重复。
