Ziegler H G, Haider S G, Passia D, Hilscher W
Andrologia. 1983 Jul-Aug;15(4):392-7. doi: 10.1111/j.1439-0272.1983.tb00159.x.
The aim of the present report was to study the course of development of rat Leydig cells from 17 fetal day (f.d.) up to 5 postnatal day (p.n.d.), with the help of enzymhistochemical reaction of delta 5-3 beta Hydroxysteroid-dehydrogenase (3 beta-HSDH). Testes were obtained from 17 to 21 days old embryos and from 1 to 5 days old offsprings. Cryostat sections were cut and processed for enzymhistochemical reaction of 3 beta-HSDH, employing dehydroepiandrosterone as substrate. The areas of the Leydig cells, showing the enzymatic activity, were measured morphometrically. The 3 beta-HSDH positive intertubular Leydig cells appear on 17 f.d., and grow further showing a maximum peak, relative to the size of the testis, on 19 f.d. Thereafter, the percentage of the Leydig cells relative to the size of the testis decreases continuously up to 4 p.n.d.. The Leydig cells did not regress during the period of observation. Postnatally, the large complexes disperse into many small complexes.
本报告的目的是借助δ5-3β-羟基类固醇脱氢酶（3β-HSDH）的酶组织化学反应，研究大鼠睾丸间质细胞从胚胎第17天（f.d.）到出生后第5天（p.n.d.）的发育过程。从17至21日龄的胚胎以及1至5日龄的后代获取睾丸。制备冰冻切片，以脱氢表雄酮为底物进行3β-HSDH的酶组织化学反应。对显示酶活性的睾丸间质细胞区域进行形态计量学测量。3β-HSDH阳性的曲精小管间睾丸间质细胞在胚胎第17天出现，并进一步生长，在胚胎第19天相对于睾丸大小达到最大峰值。此后，直至出生后第4天，睾丸间质细胞相对于睾丸大小的百分比持续下降。在观察期内，睾丸间质细胞未退化。出生后，大的细胞复合体分散成许多小的细胞复合体。
