Drainas C, Weiss R L
J Bacteriol. 1982 May;150(2):779-84. doi: 10.1128/jb.150.2.779-784.1982.
The bulk of the intracellular arginine pool in exponentially growing mycelia of Neurospora crassa is sequestered in the vacuoles. Vacuolar arginine effluxes from the vacuoles into the cytosol and is catabolized to ornithine and urea upon nitrogen starvation. The energy requirement for mobilization has been studied by treating nitrogen-starved mycelia with inhibitors or respiration or glycolysis or an uncoupler of respiration. Mobilization was inhibited by the inhibitors or the uncoupler of respiration, but not by the inhibitors of glycolysis. The inhibitors and the uncoupler of respiration reduced the ATP pool and the energy charge of the treated mycelia. The inhibitors of glycolysis reduced the ATP pool but had no effect on the energy charge. The results indicate that mobilization of arginine from the vacuoles requires metabolic energy. The forms of this energy and the mode of its association with the mobilization process are discussed.
在指数生长的粗糙脉孢菌菌丝体中，细胞内大部分精氨酸库被隔离在液泡中。液泡中的精氨酸会从液泡外流到细胞质中，并在氮饥饿时被分解代谢为鸟氨酸和尿素。通过用呼吸抑制剂、糖酵解抑制剂或呼吸解偶联剂处理氮饥饿的菌丝体，对动员所需的能量进行了研究。动员受到呼吸抑制剂或解偶联剂的抑制，但不受糖酵解抑制剂的抑制。呼吸抑制剂和解偶联剂降低了处理过的菌丝体的ATP库和能荷。糖酵解抑制剂降低了ATP库，但对能荷没有影响。结果表明，从液泡中动员精氨酸需要代谢能量。本文讨论了这种能量的形式及其与动员过程的关联方式。
