Drainas C, Weiss R L
J Bacteriol. 1982 May;150(2):770-8. doi: 10.1128/jb.150.2.770-778.1982.
The energy requirements for the uptake and retention of arginine by vacuoles of Neurospora crassa have been studied. Exponentially growing mycelial cultures were treated with inhibitors of respiration or glycolysis or an uncoupler of respiration. Catabolism of arginine was monitored as urea production in urease-less strains. The rationale was that the rate and extent of such catabolism was indicative of the cytosolic arginine concentration. No catabolism was observed in cultures treated with an inhibitor or an uncoupler of respiration, but cultures treated with inhibitors of glycolysis rapidly degraded arginine. These differences could not be accounted for by alterations in the level or activity of arginase. Mycelia growing in arginine-supplemented medium and treated with an inhibitor or uncoupler of respiration degraded an amount of arginine equivalent to the cytosolic fraction of the arginine pool. The inhibitors and the uncoupler of respiration reduced the ATP pool and the energy charge. The inhibitors of glycolysis reduced the ATP pool but did not affect the energy charge. The results suggest that metabolic energy is required for the transport of arginine into the vacuoles but not for its retention. The latter is affected by inhibitors of glycolysis. The form of energy and the nature of the vacuolar transport mechanism(s) are discussed.
对粗糙脉孢菌液泡摄取和保留精氨酸的能量需求进行了研究。用呼吸抑制剂、糖酵解抑制剂或呼吸解偶联剂处理指数生长的菌丝体培养物。在无脲酶菌株中，将精氨酸的分解代谢监测为尿素生成。其基本原理是，这种分解代谢的速率和程度表明了胞质精氨酸浓度。在用呼吸抑制剂或呼吸解偶联剂处理的培养物中未观察到分解代谢，但用糖酵解抑制剂处理的培养物迅速降解精氨酸。这些差异不能用精氨酸酶水平或活性的改变来解释。在补充精氨酸的培养基中生长并用呼吸抑制剂或呼吸解偶联剂处理的菌丝体降解的精氨酸量相当于精氨酸池的胞质部分。呼吸抑制剂和解偶联剂降低了ATP池和能荷。糖酵解抑制剂降低了ATP池，但不影响能荷。结果表明，精氨酸转运到液泡中需要代谢能量，但其保留不需要。后者受糖酵解抑制剂影响。讨论了能量形式和液泡转运机制的性质。
