Sullivan J L, Ochs H D, Wedgwood R J
J Clin Immunol. 1982 Apr;2(2):150-5. doi: 10.1007/BF00916899.
The use of a B-lymphoblastoid cell line (B-L-CL) as a stimulator cell in the mixed leukocyte reaction (MLR) was investigated in patients with immunodeficiency disorders. The kinetics of the MLR stimulated by B-L-CL are similar to those stimulated by normal allogeneic leukocytes, however, B-L-CL stimulate a greater quantitative response. Comparison of a LCL-stimulated and normal allogeneic lymphocyte-stimulated MLR in 32 patients and normal controls demonstrated that variation in the MLR was reduced when B-LCL were used as stimulator cells. The decrease in variability allowed for more sensitivity in the determination of abnormal responses; 9 of 32 patients had abnormal B-LCL-stimulated MLC responses, compared with 5 of 32 patients with abnormal responses to normal allogeneic leukocytes. Dose-response studies showed that vigorous responses could be obtained with low doses of B-LCL stimulator cells which served to better define deficient patient responses. Several patients demonstrated dissociation between the B-LCL-stimulated MLR and mitogen responses. The use of a B-LCL as a stimulator in the MLR is a valuable tool for the assessment of the immune status of patients with a variety of immunodeficiency disorders.
在免疫缺陷疾病患者中，研究了使用B淋巴细胞系（B-L-CL）作为混合淋巴细胞反应（MLR）中的刺激细胞。B-L-CL刺激的MLR动力学与正常同种异体白细胞刺激的相似，然而，B-L-CL刺激产生的定量反应更大。在32例患者和正常对照中比较LCL刺激的MLR和正常同种异体淋巴细胞刺激的MLR表明，当使用B-LCL作为刺激细胞时，MLR的变异性降低。变异性的降低使得在确定异常反应时更具敏感性；32例患者中有9例对B-LCL刺激的MLC反应异常，而对正常同种异体白细胞反应异常的32例患者中有5例。剂量反应研究表明，低剂量的B-LCL刺激细胞即可产生强烈反应，这有助于更好地界定免疫缺陷患者的反应。一些患者表现出B-LCL刺激的MLR与有丝分裂原反应之间的分离。在MLR中使用B-LCL作为刺激物是评估各种免疫缺陷疾病患者免疫状态的一种有价值的工具。
