Mortimer D, Templeton A A
J Reprod Fertil. 1982 Mar;64(2):401-8. doi: 10.1530/jrf.0.0640401.
Laparoscopic sperm recovery from the pouch of Douglas and tubal fimbriae was performed in 64 infertile couples. Spermatozoa were recovered from 16/35 couples investigated after AIH, and from 13/29 couples post coitum. The method of insemination had no effect on the result, which was positive in 45.3% of all couples, although AIH did result in significantly larger numbers of peritoneal spermatozoa. The number of peritoneal spermatozoa did not show any direct correlation with the number inseminated, but there were reductions along the tract of 5.83 (+/- 1.4 s.d.) orders of magnitude for total sperm count, and 5.52 (+/- 1.21 s.d.) for the number of motile spermatozoa. Only sperm motility had a significant influence on the success of sperm transport; spermatozoa were recovered from patients with sperm densities as low as 3.0 and 3.5 x 10(6)/ml, but with 56 and 44% motile spermatozoa. No influence of cycle day within the range +/- 4 days of ovulation on sperm transport was found. In 45 couples, routine semen analyses were apparently completely normal, but the incidence of sperm recovery was still only 49% (22/45), suggesting that a failure of sperm transport may have been a significant causative factor in their infertility.
对64对不孕夫妇进行了经腹腔镜从Douglas陷凹和输卵管伞部采集精子的操作。在人工授精后接受检查的35对夫妇中，有16对采集到了精子；在性交后接受检查的29对夫妇中，有13对采集到了精子。授精方式对结果没有影响，所有夫妇中有45.3%的结果呈阳性，尽管人工授精后腹腔内精子数量显著增多。腹腔内精子数量与授精的精子数量没有直接关联，但总精子数在整个通道上减少了5.83（±1.4标准差）个数量级，活动精子数减少了5.52（±1.21标准差）个数量级。只有精子活力对精子运输的成功有显著影响；从精子密度低至3.0和3.5×10⁶/ml但活动精子分别为56%和44%的患者中采集到了精子。未发现排卵日前后±4天范围内的月经周期日对精子运输有影响。在45对夫妇中，常规精液分析显然完全正常，但精子采集成功率仍仅为49%（22/45），这表明精子运输障碍可能是他们不孕的一个重要原因。
