Mortimer D, Leslie E E, Kelly R W, Templeton A A
J Reprod Fertil. 1982 Mar;64(2):391-9. doi: 10.1530/jrf.0.0640391.
Light microscopic assessment of human spermatozoa in post-coital samples of cervical mucus revealed a significant improvement in the general sperm morphology between the semen and the cervix. Further analysis showed that the excluded spermatozoa were more likely to be those with midpiece or tail defects that impaired motility. Significant changes were also found when the morphology of spermatozoa recovered from the uterus and Fallopian tubes following AIH was compared with the semen used for insemination: in the semen, uterus and oviducts there were respectively 53, 77 and 71% spermatozoa with completely normal morphology, and 12, 3 and 0.6% spermatozoa with defects of the midpiece and/or tail (as assessed by surface replica electron microscopy). Selection of spermatozoa in vitro by allowing them to swim upwards through a nickel mesh also reduced the number of spermatozoa with abnormal morphology, particularly of the midpiece and tail. It is concluded that the apparent selection of morphologically normal spermatozoa is not a direct function of the female tract, but that the spermatozoa can effect their own selection because of their differential motility.
对性交后宫颈黏液样本中的人类精子进行光学显微镜评估发现，精液中的精子与宫颈中的精子相比，总体精子形态有显著改善。进一步分析表明，被排除的精子更可能是那些具有中段或尾部缺陷且影响活力的精子。当将人工授精后从子宫和输卵管中回收的精子形态与用于授精的精液进行比较时，也发现了显著变化：在精液、子宫和输卵管中，形态完全正常的精子分别占53%、77%和71%，中段和/或尾部有缺陷的精子分别占12%、3%和0.6%（通过表面复型电子显微镜评估）。通过让精子向上游过镍网在体外选择精子，也减少了形态异常的精子数量，尤其是中段和尾部异常的精子。得出的结论是，形态正常精子的明显选择并非女性生殖道的直接作用，而是精子因其不同的活力能够实现自身选择。
