λ dv DNA的体外复制。
Replication of lambda dv DNA in vitro.
作者信息
Anderl A, Klein A
出版信息
Nucleic Acids Res. 1982 Mar 11;10(5):1733-40. doi: 10.1093/nar/10.5.1733.
Abstract
Exogenous lambda dv DNA was replicated in extracts prepared from E. coli cells carrying plasmids with inducible lambda O and /or P genes. Extracts from cells carrying only one of the two lambda replication functions complement each other in this reaction. The reaction further requires ribonucleotide triphosphates, an ATP regenerating system, DNA gyrase and RNA polymerase functions. Density labelling of the superhelical reaction products results in hybrid density indicating that one complete round of replication has taken place in vitro.
摘要
外源λ dv DNA 在从携带具有可诱导λ O 和/或 P 基因的质粒的大肠杆菌细胞制备的提取物中进行复制。携带两种λ复制功能之一的细胞提取物在该反应中相互补充。该反应还需要核糖核苷三磷酸、ATP 再生系统、DNA 回旋酶和 RNA 聚合酶功能。超螺旋反应产物的密度标记产生杂合密度，表明在体外已经发生了一轮完整的复制。
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