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1
Replication of lambda dv DNA in vitro.λ dv DNA的体外复制。
Nucleic Acids Res. 1982 Mar 11;10(5):1733-40. doi: 10.1093/nar/10.5.1733.
2
Initiation of bacteriophage lambda DNA replication in vitro with purified lambda replication proteins.利用纯化的λ噬菌体复制蛋白在体外启动λ噬菌体DNA复制。
Proc Natl Acad Sci U S A. 1982 Oct;79(20):6176-80. doi: 10.1073/pnas.79.20.6176.
3
Replication of lambda dv plasmid in vitro promoted by purified lambda O and P proteins.纯化的λ O和P蛋白促进λ dv质粒的体外复制。
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7639-43. doi: 10.1073/pnas.79.24.7639.
4
The bacteriophage lambda O and P protein initiators promote the replication of single-stranded DNA.噬菌体λ O和P蛋白引发剂促进单链DNA的复制。
Nucleic Acids Res. 1984 Apr 11;12(7):3069-88. doi: 10.1093/nar/12.7.3069.
5
Multiple initiation sites of DNA replication flanking the origin region of lambda dv genome.λdv基因组起源区域侧翼的多个DNA复制起始位点。
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7402-6. doi: 10.1073/pnas.81.23.7402.
6
The bacteriophage lambda O replication protein: isolation and characterization of the amplified initiator.噬菌体λO复制蛋白：扩增启动子的分离与鉴定
Nucleic Acids Res. 1983 Nov 11;11(21):7435-52.
7
Initiation of DNA replication on single-stranded DNA templates catalyzed by purified replication proteins of bacteriophage lambda and Escherichia coli.由噬菌体λ和大肠杆菌的纯化复制蛋白催化的单链DNA模板上DNA复制的起始
Proc Natl Acad Sci U S A. 1985 Jun;82(12):3988-92. doi: 10.1073/pnas.82.12.3988.
8
Origin and initiation sites of lambda dv DNA replication in vitro.λ dv DNA体外复制的起源和起始位点。
Basic Life Sci. 1985;30:151-72. doi: 10.1007/978-1-4613-2447-8_15.
9
Escherichia coli dnaA initiation function is required for replication of plasmids derived from coliphage lambda.大肠杆菌dnaA起始功能是源自λ噬菌体的质粒复制所必需的。
J Mol Biol. 1987 Nov 20;198(2):203-10. doi: 10.1016/0022-2836(87)90306-8.
10
Role of an autorepression system in the control of lambda dv plasmid copy number and incompatibility.自我抑制系统在λ dv质粒拷贝数控制及不相容性中的作用。
Mol Gen Genet. 1980;179(3):509-19. doi: 10.1007/BF00271740.

引用本文的文献

1
A CI-independent form of replicative inhibition: turn off of early replication of bacteriophage lambda.一种与复制起始无关的复制抑制形式：噬菌体 λ 的早期复制被关闭。
PLoS One. 2012;7(5):e36498. doi: 10.1371/journal.pone.0036498. Epub 2012 May 10.
2
Replication of the broad host range plasmid RSF1010 in cell-free extracts of Escherichia coli and Pseudomonas aeruginosa.广宿主范围质粒RSF1010在大肠杆菌和铜绿假单胞菌无细胞提取物中的复制
Nucleic Acids Res. 1982 Aug 11;10(15):4687-702. doi: 10.1093/nar/10.15.4687.
3
The bacteriophage lambda O and P protein initiators promote the replication of single-stranded DNA.噬菌体λ O和P蛋白引发剂促进单链DNA的复制。
Nucleic Acids Res. 1984 Apr 11;12(7):3069-88. doi: 10.1093/nar/12.7.3069.
4
Structure and function of mutants in the P gene of bacteriophage lambda leading to the pi phenotype.导致π表型的噬菌体λ P基因中突变体的结构与功能
Mol Gen Genet. 1983;192(3):430-5. doi: 10.1007/BF00392186.
5
The bacteriophage lambda O replication protein: isolation and characterization of the amplified initiator.噬菌体λO复制蛋白：扩增启动子的分离与鉴定
Nucleic Acids Res. 1983 Nov 11;11(21):7435-52.
6
Interactions of bacteriophage and host macromolecules in the growth of bacteriophage lambda.噬菌体λ生长过程中噬菌体与宿主大分子的相互作用
Microbiol Rev. 1984 Dec;48(4):299-325. doi: 10.1128/mr.48.4.299-325.1984.
7
Initiation of bacteriophage lambda DNA replication in vitro with purified lambda replication proteins.利用纯化的λ噬菌体复制蛋白在体外启动λ噬菌体DNA复制。
Proc Natl Acad Sci U S A. 1982 Oct;79(20):6176-80. doi: 10.1073/pnas.79.20.6176.
8
Initiation of plasmid R1 replication in vitro is independent of transcription by host RNA polymerase.质粒R1在体外的复制起始不依赖于宿主RNA聚合酶的转录。
Nucleic Acids Res. 1984 Jul 11;12(13):5175-91. doi: 10.1093/nar/12.13.5175.
9
Role of the Escherichia coli DnaK and DnaJ heat shock proteins in the initiation of bacteriophage lambda DNA replication.大肠杆菌DnaK和DnaJ热休克蛋白在噬菌体λ DNA复制起始中的作用。
Proc Natl Acad Sci U S A. 1988 Sep;85(18):6632-6. doi: 10.1073/pnas.85.18.6632.
10
The role of template superhelicity in the initiation of bacteriophage lambda DNA replication.模板超螺旋在噬菌体λ DNA 复制起始中的作用。
Nucleic Acids Res. 1988 Oct 25;16(20):9611-30. doi: 10.1093/nar/16.20.9611.

本文引用的文献

1
STUDIES IN THE PHYSIOLOGICAL GENETICS OF SOME SUPPRESSOR-SENSITIVE MUTANTS OF BACTERIOPHAGE LAMBDA.噬菌体λ某些抑制敏感突变体的生理遗传学研究
Virology. 1965 Jul;26:489-99. doi: 10.1016/0042-6822(65)90011-5.
2
Plasmid R1 DNA replication dependent on protein synthesis in cell-free extracts of E. coli.质粒R1 DNA复制依赖于大肠杆菌无细胞提取物中的蛋白质合成。
Nature. 1981 Jan 22;289(5795):326-8. doi: 10.1038/289326a0.
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Synthesis and decay of lambda DNA replication proteins in minicells.
Biochem Biophys Res Commun. 1980 Jan 15;92(1):120-6. doi: 10.1016/0006-291x(80)91528-4.
4
Purified bacteriophage lambda O protein binds to four repeating sequences at the lambda replication origin.
Nucleic Acids Res. 1981 Apr 24;9(8):1789-99. doi: 10.1093/nar/9.8.1789.
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Cloning of the replication gene O of E. coli bacteriophage lambda and its expression under the control of the lac promoter.
Gene. 1980 Aug;10(3):195-203. doi: 10.1016/0378-1119(80)90049-9.
6
Isolation of a complex between the P protein of phage lambda and the dnaB protein of Escherichia coli.噬菌体λ的P蛋白与大肠杆菌的dnaB蛋白之间复合物的分离。
Eur J Biochem. 1980 Mar;105(1):1-6. doi: 10.1111/j.1432-1033.1980.tb04467.x.
7
Studies on the functions of DNA helicase I and DNA helicase II of Escherichia coli.大肠杆菌DNA解旋酶I和DNA解旋酶II功能的研究。
J Biol Chem. 1980 Oct 25;255(20):9746-52.
8
DNA replication and messenger RNA production after induction of wild-type lambda bacteriophage and lambda mutants.野生型λ噬菌体和λ突变体诱导后的DNA复制及信使核糖核酸的产生
J Mol Biol. 1966 Aug;19(1):174-86. doi: 10.1016/s0022-2836(66)80059-1.
9
Strains of phage lambda in current use.目前使用的λ噬菌体菌株。
Virology. 1969 Jun;38(2):349-51. doi: 10.1016/0042-6822(69)90378-x.
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Stability of lambda O and P replication functions.
Virology. 1974 Mar;58(1):313-5. doi: 10.1016/0042-6822(74)90168-8.

λ dv DNA的体外复制。

Replication of lambda dv DNA in vitro.

作者信息

Anderl A, Klein A

出版信息

Nucleic Acids Res. 1982 Mar 11;10(5):1733-40. doi: 10.1093/nar/10.5.1733.

DOI:10.1093/nar/10.5.1733
PMID:6461846
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320562/
Abstract

Exogenous lambda dv DNA was replicated in extracts prepared from E. coli cells carrying plasmids with inducible lambda O and /or P genes. Extracts from cells carrying only one of the two lambda replication functions complement each other in this reaction. The reaction further requires ribonucleotide triphosphates, an ATP regenerating system, DNA gyrase and RNA polymerase functions. Density labelling of the superhelical reaction products results in hybrid density indicating that one complete round of replication has taken place in vitro.

摘要

外源λ dv DNA 在从携带具有可诱导λ O 和/或 P 基因的质粒的大肠杆菌细胞制备的提取物中进行复制。携带两种λ复制功能之一的细胞提取物在该反应中相互补充。该反应还需要核糖核苷三磷酸、ATP 再生系统、DNA 回旋酶和 RNA 聚合酶功能。超螺旋反应产物的密度标记产生杂合密度，表明在体外已经发生了一轮完整的复制。