广宿主范围质粒RSF1010在大肠杆菌和铜绿假单胞菌无细胞提取物中的复制
Replication of the broad host range plasmid RSF1010 in cell-free extracts of Escherichia coli and Pseudomonas aeruginosa.
作者信息
Diaz R, Staudenbauer W L
出版信息
Nucleic Acids Res. 1982 Aug 11;10(15):4687-702. doi: 10.1093/nar/10.15.4687.
Abstract
Replication of exogenous RSF1010 DNA can be carried out by soluble enzyme systems from Escherichia coli and Pseudomonas aeruginosa. It requires the function of RSF1010-encoded replication protein(s), which is not expressed in extracts from plasmid-free bacteria. In contrast to previously described in vitro systems for plasmid replication, initiation of RSF1010 DNA synthesis is independent of transcription catalyzed by host RNA polymerase. This is indicated by the insensitivity of RSF1010 replication to rifampicin as well as to RNA polymerase antibodies. It is proposed that a host RNA polymerase transcription-independent initiation mechanism might be a general property of broad host range plasmids.
摘要
外源RSF1010 DNA的复制可通过来自大肠杆菌和铜绿假单胞菌的可溶性酶系统来进行。它需要RSF1010编码的复制蛋白发挥功能，而该蛋白在无质粒细菌的提取物中不表达。与先前描述的质粒复制体外系统不同，RSF1010 DNA合成的起始不依赖于宿主RNA聚合酶催化的转录。这通过RSF1010复制对利福平以及RNA聚合酶抗体不敏感得以表明。有人提出，宿主RNA聚合酶转录非依赖性起始机制可能是广泛宿主范围质粒的一个普遍特性。
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