Bolaños A, Lorenzo A
Rev Esp Fisiol. 1984 Mar;40(1):117-22.
Simultaneous measurements of the transmural potential difference (PD) and the short-circuit current intensity (Isc) in the posterior intestine of the fish Blennius parvicornis were made in normal Ringer and in solutions of different ionic composition. The ouabain effects on these two parameters were also tested in normal Ringer solution. The absence of K+ from the Ringer solution on both the mucosal and serosal sides has no apparent effect on the PD and Isc within the first 15 min, but it makes them null after 30 min. When Na+ is substituted in both compartments, using Tris as substitute, a serosal negativity increase is initially observed, but it gradually decreases to zero after 30 min of experimentation. Similarly the PD and Isc drop to zero in the absence of Cl- (sulfate as substitute). Ouabain diminishes the serosa negative potential difference to zero after 30 min presenting a lineal relation to the Isc. A likely transport mechanism for Cl- dependent on the Na+ - K+ pump, is discussed.
在正常任氏液和不同离子组成的溶液中，对小角鳚鱼后肠的跨壁电位差（PD）和短路电流强度（Isc）进行了同步测量。还在正常任氏液中测试了哇巴因对这两个参数的影响。任氏液黏膜侧和浆膜侧均无K⁺时，最初15分钟内对PD和Isc无明显影响，但30分钟后它们变为零。当两个隔室都用Tris替代Na⁺时，最初观察到浆膜负性增加，但实验30分钟后逐渐降至零。同样，在没有Cl⁻（用硫酸盐替代）的情况下，PD和Isc降至零。哇巴因在30分钟后将浆膜负电位差降至零，与Isc呈线性关系。讨论了一种可能依赖于Na⁺ - K⁺泵的Cl⁻转运机制。
