Badía P, Lorenzo A
Rev Esp Fisiol. 1982 Jun;38(2):221-6.
The electrical difference (PD) and short-circuit current (Isc) across anterior intestine have been studied in vitro with solutions of different ionic composition in Gobius maderensis. 30 minutes after the beginning of the experiment a PD of about 2 mv negative serosa and an Isc of approximately 200 micro A/cm2 were recorded. Omission of sodium (Tris as substitute) from the luminal side or from both sides leads to an increment of 800% in PD more negative serosa together with an increase of 969% in Isc. In the absence of chloride (sulphate as substitute) the PD and Isc decreased and attained constant positive values. When the potassium was omitted from the luminal side or from both sides neither the P nor the Isc changed. These findings corroborate the assumption that active transport of chloride ions from mucosa to serosa is the major factor for transmural PD and account for the short-circuit current in the anterior intestine of Gobius maderensis.
在体外，使用不同离子组成的溶液对马德拉岛鳚（Gobius maderensis）的前肠跨膜电位差（PD）和短路电流（Isc）进行了研究。实验开始30分钟后，记录到浆膜侧约2毫伏的负跨膜电位差和大约200微安/平方厘米的短路电流。从肠腔侧或两侧去除钠（用Tris替代）会导致浆膜侧更负的跨膜电位差增加800%，同时短路电流增加969%。在没有氯离子（用硫酸根替代）的情况下，跨膜电位差和短路电流降低并达到恒定的正值。当从肠腔侧或两侧去除钾时，跨膜电位差和短路电流均未改变。这些发现证实了以下假设，即氯离子从黏膜到浆膜的主动转运是跨膜电位差的主要因素，并解释了马德拉岛鳚前肠的短路电流。
