Neuweiler J, Ragaz A, Keller R
Schweiz Med Wochenschr. 1984 Jun 23;114(25):907-9.
Different results have been observed in systematically analysing bacteriological cultures of (a) bronchial secretions aspirated during fiberoptic bronchoscopy and (b) of sputum. In a prospective study of 23 patients with chronic infections of the lower respiratory tract, bacteriological cultures of the sputum showed more bronchopathogenic strains than those of subsequently removed bronchial secretions. These different findings may be attributed to a bactericidal effect of topical anesthetics used during bronchoscopy. To evaluate the influence of anesthetics on the growth of microorganisms, several strains cultured from sputum specimens were exposed to various solutions of the most commonly used anesthetics. The growth of Haemophilus influenzae and Diplococcus pneumoniae was completely depressed by all the tested solutions and dilutions. Only Staphylococcus aureus and several strains of Enterobacteriaceae were resistant even to high concentrations of anesthetic solutions. The results suggest that specimens of bronchial secretions taken by routine fiberoptic techniques cannot be used for reliable bacteriological diagnosis of lower respiratory tract infections.
在对（a）纤维支气管镜检查期间吸出的支气管分泌物和（b）痰液进行系统的细菌学培养分析时，观察到了不同的结果。在一项对23例下呼吸道慢性感染患者的前瞻性研究中，痰液的细菌学培养显示，支气管致病菌株比随后采集的支气管分泌物中的更多。这些不同的发现可能归因于支气管镜检查期间使用的局部麻醉剂的杀菌作用。为了评估麻醉剂对微生物生长的影响，将从痰液标本中培养的几种菌株暴露于最常用麻醉剂的各种溶液中。所有测试溶液和稀释液都完全抑制了流感嗜血杆菌和肺炎双球菌的生长。即使对高浓度的麻醉剂溶液，只有金黄色葡萄球菌和几种肠杆菌科菌株具有抗性。结果表明，通过常规纤维支气管镜技术采集的支气管分泌物标本不能用于下呼吸道感染的可靠细菌学诊断。
