Holschneider A M, Amano S, Urban A, Donhauser G
Z Kinderchir. 1984 Jun;39(3):182-90. doi: 10.1055/s-2008-1044206.
Free autologous muscle transplants were performed on 59 Wistar rats of 70 to 90 days of age according to the method described by Schmidt (1978). The free smooth muscular transplant was prestretched by 0%, 100%, 120%, 150% and 200% and the animals were sacrificed on the 5th, 10th, 20th, 40th and 60th postoperative day, respectively. Five animals died postoperatively, of these three during ileus and stenosis after more than 150% prestretching of the transplant. Complications occurred in eleven further animals, which included again ileus phenomena nine times due to stenosing or disturbance of defecation, also as a result of obstruction phenomena. Complications always occurred in animals with more than 150% transplant prestretching. No relaxation reflex was observed manometrically in any of the transplants. Budding of nerve fibres also did not occur in any of the cases, neither from the plexus myentericus of the inner intestinal wall nor from outside. Histology revealed already after five days' survival leucocytous infiltration and connective tissue multiplication which increased with increasing prestretching of the transplant and longer survival time of the animals until complete metaplasia of the connective tissue. In preparations with 100 up to 120% prestretch not more than 20% of the original muscular mass was preserved. With prestretching of 150% and more the transplant underwent complete fibrosis. On the basis of these studies we must discourage performance of free transplants of smooth-muscle colon segments in newborn and young infants.
根据施密特（1978年）描述的方法，对59只70至90日龄的Wistar大鼠进行了自体游离肌肉移植。游离平滑肌移植分别预拉伸0%、100%、120%、150%和200%，并分别在术后第5天、第10天、第20天、第40天和第60天处死动物。术后有5只动物死亡，其中3只在移植预拉伸超过150%后出现肠梗阻和狭窄。另有11只动物出现并发症，其中9次因狭窄或排便障碍再次出现肠梗阻现象，也是由梗阻现象引起的。并发症总是发生在移植预拉伸超过150%的动物身上。在任何移植中均未通过压力测量观察到松弛反射。在任何情况下，神经纤维的出芽也未发生，既未从肠壁内层的肌间神经丛长出，也未从外部长出。组织学显示，存活5天后即出现白细胞浸润和结缔组织增生，随着移植预拉伸程度的增加和动物存活时间的延长，结缔组织增生直至完全化生。在预拉伸100%至120%的标本中，保留的原始肌肉质量不超过20%。预拉伸150%及以上时，移植物完全纤维化。基于这些研究，我们不鼓励对新生儿和幼儿进行平滑肌结肠段的游离移植。
