Al-Sheikh W, Heal A V, Pefkaros K C, Pina I L, Serafini A N, Ihmedian I H, Ashkar F S
Am J Cardiol. 1984 Aug 1;54(3):269-73. doi: 10.1016/0002-9149(84)90180-2.
Clinical evaluation of patient's symptoms, electrocardiographic changes and increased serum enzyme levels, specifically creatine kinase (CK)-MB by electrophoresis, are established as the primary diagnostic indicators for myocardial infarction (MI). Two hundred fifteen patients were evaluated in this study. Of these patients, 102 were admitted to the coronary care unit and 113 were admitted to the emergency room and screened for possible MI. The immunoradiometric assay used in this study was a double antibody "sandwich" technique, which utilizes antibody to the M and B monomers of the CK isoenzymes. This assay is specific for the CK-MB isoenzyme, which is present in increased levels in MI. The intraassay coefficients of variation for 30 samples were 11.7% (mean 4.1 equivalent units [EU]/liter) and 8.4% (mean 15.4 EU/liter) and the interassay coefficients of variation for 30 samples were 11.1% (mean 2.6 EU/liter) and 8.1% (mean 13.6 EU/liter). The diagnostic sensitivity, specificity and accuracy in this study was 100%, respectively. The CK-MB by the immunoradiometric assay was found to be significantly more accurate than electrophoresis and, therefore, a reliable and also technically simpler replacement for electrophoresis.
患者症状、心电图变化以及血清酶水平升高（特别是通过电泳检测的肌酸激酶（CK）-MB）的临床评估已被确立为心肌梗死（MI）的主要诊断指标。本研究对215名患者进行了评估。在这些患者中，102人被收治入冠心病监护病房，113人被收治入急诊室并接受了可能的MI筛查。本研究中使用的免疫放射分析是一种双抗体“夹心”技术，它利用针对CK同工酶M和B单体的抗体。该分析对CK-MB同工酶具有特异性，在MI中其水平会升高。30个样本的批内变异系数分别为11.7%（平均4.1等效单位[EU]/升）和8.4%（平均15.4 EU/升），30个样本的批间变异系数分别为11.1%（平均2.6 EU/升）和8.1%（平均13.6 EU/升）。本研究中的诊断敏感性、特异性和准确性分别为100%。通过免疫放射分析检测的CK-MB被发现比电泳显著更准确，因此是一种可靠且技术上更简单的电泳替代方法。
