Schneeberger E E, Hamelin M
Am J Physiol. 1984 Aug;247(2 Pt 2):H206-17. doi: 10.1152/ajpheart.1984.247.2.H206.
To examine directly the interaction of circulating proteins (CP) with the glycocalyx of pulmonary endothelium and its effect on endothelial permeability, two types of experiments were carried out. In the first, rats were exchange transfused with graded amounts of FC-43 fluorocarbon emulsion (FCE) resulting in CP concentrations of 25, 10, and 4 mg/ml, respectively. In the second, rats were exchange transfused with FCE to remove 99.9% of CP. The rats were then exchange transfused with 1 ml FCE containing 60 mg/ml of rat serum protein and killed 3.5, 7.5, and 15 min after the administration of protein. In all animals the distribution of albumin and immunoglobulin G (IgG) was visualized by immunocytochemistry, and endothelial permeability to native ferritin was measured by morphometry. In the depletion experiments increased endothelial permeability to ferritin coincided with loss of adsorbed albumin and IgG from the glycocalyx. Conversely, the presence of administered serum proteins in the glycocalyx and in a few luminal vesicles was associated with an endothelial permeability to ferritin indistinguishable from that of controls. These observations suggest that the adsorption of CP to the endothelial glycocalyx renders the underlying endothelium less permeable to ferritin.
为了直接研究循环蛋白（CP）与肺内皮细胞糖萼的相互作用及其对内皮通透性的影响，进行了两种类型的实验。在第一个实验中，用不同剂量的FC - 43氟碳乳剂（FCE）对大鼠进行换血，使CP浓度分别达到25、10和4mg/ml。在第二个实验中，用FCE对大鼠进行换血以去除99.9%的CP。然后用含有60mg/ml大鼠血清蛋白的1ml FCE对大鼠进行换血，并在给予蛋白质后3.5、7.5和15分钟处死大鼠。在所有动物中，通过免疫细胞化学观察白蛋白和免疫球蛋白G（IgG）的分布，并通过形态计量学测量内皮对天然铁蛋白的通透性。在耗竭实验中，内皮对铁蛋白的通透性增加与糖萼上吸附的白蛋白和IgG的丢失同时发生。相反，糖萼和一些管腔小泡中存在给予的血清蛋白与内皮对铁蛋白的通透性有关，与对照组无明显差异。这些观察结果表明，CP吸附到内皮糖萼上使下层内皮对铁蛋白的通透性降低。
