Peladan F, Monteil H
Ann Microbiol (Paris). 1984 May-Jun;135A(3):411-25.
Gas liquid-chromatography was used in order to characterize the volatile fatty acids produced in culture supernatants by 632 Pseudomonas strains. Statistical analysis of these results allowed testing of the discriminatory power of this analytical methodology (factor analysis) to point out the presence of subgroups (clustering according to the variance) and to show the relationship between species and subspecies (three-dimensional plot). Fourteen Pseudomonas species could be accurately characterized by this methodology; the classification of Pseudomonas build up, on the basis of qualitative and quantitative aspects of their volatile fatty acids production, agrees with the current classification based on rRNA/DNA homology complexes.
采用气液色谱法对632株假单胞菌菌株在培养上清液中产生的挥发性脂肪酸进行表征。对这些结果进行统计分析,以检验这种分析方法(因子分析)的鉴别能力,从而指出亚组的存在(根据方差进行聚类),并显示物种和亚种之间的关系(三维图)。通过这种方法可以准确地表征14种假单胞菌;基于挥发性脂肪酸产生的定性和定量方面对假单胞菌进行的分类,与目前基于rRNA/DNA同源复合物的分类一致。