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乳酸克鲁维酵母氯霉素抗性突变体的分离及融合杂种有丝分裂分离分析的表征

Isolation of chloramphenicol-resistant mutants of Kluyveromyces lactis and characterization by mitotic segregation analysis of fused hybrids.

作者信息

Allmark B M

出版信息

Antonie Van Leeuwenhoek. 1984;50(2):167-75. doi: 10.1007/BF00400178.

DOI:10.1007/BF00400178
PMID:6465878
Abstract

Chloramphenicol-resistant mutants of Kluyveromyces lactis were isolated following manganese mutagenesis. Mutants were characterized biochemically in order to determine the effect of CAP on respiration. The genetic basis of the resistance was determined using mitotic segregation analysis of fused hybrids where the transmission of mitochondrial genes from a parent in each parasexual cross was impaired by treatment with ethidium bromide.

摘要

在经锰诱变后，分离出了乳酸克鲁维酵母的氯霉素抗性突变体。对突变体进行了生化特性分析，以确定氯霉素对呼吸作用的影响。利用融合杂种的有丝分裂分离分析确定抗性的遗传基础，在每个准性杂交中，亲本线粒体基因的传递通过溴化乙锭处理而受到损害。

相似文献

1
Isolation of chloramphenicol-resistant mutants of Kluyveromyces lactis and characterization by mitotic segregation analysis of fused hybrids.乳酸克鲁维酵母氯霉素抗性突变体的分离及融合杂种有丝分裂分离分析的表征
Antonie Van Leeuwenhoek. 1984;50(2):167-75. doi: 10.1007/BF00400178.
2
Extrachromosomal genetics in the yeast Kluyveromyces lactis. Isolation and characterization of antimycin-resistant mutants.乳酸克鲁维酵母中的染色体外遗传学。抗霉素抗性突变体的分离与鉴定。
Curr Genet. 1987;11(6-7):475-82. doi: 10.1007/BF00384609.
3
Extrachromosomal inheritance in Schizosaccharomyces pombe. IV. Isolation and genetic characterization of mutants resistant to chloramphenicol and erythromycin using the mutator properties of mutant anar-8.粟酒裂殖酵母中的染色体外遗传。IV. 利用突变体anar-8的诱变特性分离对氯霉素和红霉素耐药的突变体并进行遗传特征分析。
Mol Gen Genet. 1977 Oct 24;155(3):339-46. doi: 10.1007/BF00272814.
4
Biosynthesis of yeast mitochondria. IV. Antibiotic effects on growth, cytochrome synthesis, and respiration in Kluyveromyces lactis.酵母线粒体的生物合成。IV. 抗生素对乳酸克鲁维酵母生长、细胞色素合成及呼吸作用的影响。
Mol Gen Genet. 1978 Aug 17;164(2):185-93. doi: 10.1007/BF00267383.
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Autorepression of ethidium bromide mutagenesis in Kluyveromyces lactis, a petite-negative yeast.乳酸克鲁维酵母（一种小菌落阴性酵母）中溴化乙锭诱变的自动抑制
Biochem Soc Trans. 1977;5(1):264-7. doi: 10.1042/bst0050264.
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Isolation and biochemical characteristics of petite mutants of Kluyveromyces lactis [proceedings].
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The isolation and genetic characterization of extrachromosomal chloramphenicol and oligomycin-resistant mutants from the petite-negative yeast Kluyveromyces lactis.从小体型阴性酵母乳酸克鲁维酵母中分离出染色体外氯霉素和寡霉素抗性突变体并进行遗传特征分析。
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Intergeneric transfer of deoxyribonucleic acid killer plasmids, pGKl1 and pGKl2, from Kluyveromyces lactis into Saccharomyces cerevisiae by cell fusion.通过细胞融合将脱氧核糖核酸杀伤质粒pGKl1和pGKl2从乳酸克鲁维酵母属转移至酿酒酵母属。
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[Eremothecium ashbyii mutants resistant to 8-azaguanine. III. Nucleotide pyrophosphorylase activity of mutants with a varying degree of 8-azaguanine resistance].[阿舒假囊酵母8-氮杂鸟嘌呤抗性突变体。III. 不同程度8-氮杂鸟嘌呤抗性突变体的核苷酸焦磷酸化酶活性]
Genetika. 1982 May;18(5):732-5.

本文引用的文献

1
Protoplast fusion in a petite-negative yeast, Kluyveromyces lactis.原生质体融合在 petite-negative 酵母，乳酸克鲁维酵母中。
Curr Genet. 1980 Jul;2(1):87-93. doi: 10.1007/BF00445699.
2
Allele specific determinants of homothallism in Saccharomyces lactis.乳酸克鲁维酵母同宗配合的等位基因特异性决定因素。
Genetics. 1966 Apr;53(4):727-40. doi: 10.1093/genetics/53.4.727.
3
Respiratory-deficient mutants in Saccharomyces lactis.乳酸克鲁维酵母中的呼吸缺陷型突变体。
J Bacteriol. 1968 Aug;96(2):457-61. doi: 10.1128/jb.96.2.457-461.1968.
4
Mitochondrial genetics. I. Methodology and phenomenology.线粒体遗传学。I. 方法学与现象学。
Symp Soc Exp Biol. 1970;24:449-96.
5
Biosynthesis of yeast mitochondria. Drug effects on the petite negative yeast kluyveromyces lactis.酵母线粒体的生物合成。药物对小菌落阴性酵母乳酸克鲁维酵母的影响。
Biochem Biophys Res Commun. 1971 Jul 16;44(2):396-402. doi: 10.1016/0006-291x(71)90613-9.
6
Isolation and characterization of respiratory-deficient mutants of Kluyveromyces lactis, a petite-negative yeast [proceedings].
Biochem Soc Trans. 1977;5(5):1498-500. doi: 10.1042/bst0051498.
7
The isolation and genetic characterization of extrachromosomal chloramphenicol and oligomycin-resistant mutants from the petite-negative yeast Kluyveromyces lactis.从小体型阴性酵母乳酸克鲁维酵母中分离出染色体外氯霉素和寡霉素抗性突变体并进行遗传特征分析。
Mol Gen Genet. 1977 Apr 29;152(3):183-91. doi: 10.1007/BF00268816.
8
Protoplast fusion of Schizosaccharomyces pombe Auxotrophic mutants of identical mating-type.相同交配型粟酒裂殖酵母营养缺陷型突变体的原生质体融合。
Mol Gen Genet. 1977 Feb 28;151(1):77-81. doi: 10.1007/BF00446915.
9
Fusion of mitochondria with protoplasts in Saccharomyces cerevisiae.酿酒酵母中线粒体与原生质体的融合
Mol Gen Genet. 1979 Mar 5;170(3):243-7. doi: 10.1007/BF00267057.
10
Uniparental inheritance of mitochondrial genes in yeast: dependence on input bias of mitochondrial DNA and preliminary investigations of the mechanism.酵母中线粒体基因的单亲遗传：对线粒体DNA输入偏差的依赖性及机制的初步研究
Genetics. 1978 Aug;89(4):615-51. doi: 10.1093/genetics/89.4.615.