Blaszczyk M, Hansson G C, Karlsson K A, Larson G, Stromberg N, Thurin J, Herlyn M, Steplewski Z, Koprowski H
Arch Biochem Biophys. 1984 Aug 15;233(1):161-8. doi: 10.1016/0003-9861(84)90612-x.
Monoclonal antibodies directed against human cancer cells were prepared by the murine hybridoma technique. These antibodies detect Lewis blood group antigens as determined by indirect solid-phase radioimmunoassay, hapten inhibition studies, and chromatogram binding assay. One monoclonal antibody is specific for the Lea terminal carbohydrate of Gal beta 1----3Glc NAc(4----1 alpha Fuc) beta 1----3LacCer. Five monoclonal antibodies react with the Leb terminal carbohydrate sequence of Fuc alpha 1----2Gal beta 1----3GlcNAc(4----1 alpha Fuc) beta 1----3LacCer, and four of these antibodies are highly specific for this glycolipid and do not react with other similar di- and monofucosylated glycolipids. One of the anti-Leb antibodies cross-reacts with blood group H glycolipid and has binding properties similar to those of the previously described antibody NS-10-17 [M. Brockhaus, J. L. Magnani, M. Blaszczyk, Z. Steplewski, H. Koprowski, K.-A. Karlsson, G. Larson, and V. Ginsburg (1981) J. Biol. Chem. 256, 13223-13225]. Two antibodies react with both the Lea and Leb antigens, though both bind preferentially to Leb.
采用鼠杂交瘤技术制备了针对人癌细胞的单克隆抗体。通过间接固相放射免疫测定、半抗原抑制研究和色谱结合测定确定，这些抗体可检测刘易斯血型抗原。一种单克隆抗体对Galβ1----3GlcNAc(4----1αFuc)β1----3LacCer的Lea末端碳水化合物具有特异性。五种单克隆抗体与Fucα1----2Galβ1----3GlcNAc(4----1αFuc)β1----3LacCer的Leb末端碳水化合物序列发生反应，其中四种抗体对这种糖脂具有高度特异性，不与其他类似的二岩藻糖基化和单岩藻糖基化糖脂发生反应。一种抗Leb抗体与血型H糖脂发生交叉反应，其结合特性与先前描述的抗体NS-10-17 [M. Brockhaus, J. L. Magnani, M. Blaszczyk, Z. Steplewski, H. Koprowski, K.-A. Karlsson, G. Larson, and V. Ginsburg (1981) J. Biol. Chem. 256, 13223-13225]相似。两种抗体与Lea和Leb抗原均发生反应，不过两者都优先结合Leb。
