镁离子对牛脑和猪肝谷氨酸脱氢酶与ATP及GTP相互作用的影响。
The effects of magnesium ions on the interactions of ox brain and liver glutamate dehydrogenase with ATP and GTP.
作者信息
McCarthy A D, Tipton K F
出版信息
Biochem J. 1984 Jun 15;220(3):853-5. doi: 10.1042/bj2200853.
Abstract
The effects of ATP and GTP on the activities of ox liver and brain glutamate dehydrogenase were determined in the absence and presence of added Mg2+ ions. Although GTP was an inhibitor of the enzyme reaction assayed in the direction of NAD+ reduction, the magnesium complex of this nucleotide had no effect on the activity. Similarly the magnesium complex of ATP was without effect on the activity of the enzyme although the free nucleotide was an activator. These results suggest that it is important to take account of magnesium complex formation when considering the regulatory actions of these nucleotides.
摘要
在添加和未添加Mg2+离子的情况下，测定了ATP和GTP对牛肝和脑谷氨酸脱氢酶活性的影响。尽管GTP在以NAD+还原方向测定的酶反应中是一种抑制剂，但该核苷酸的镁络合物对活性没有影响。同样，ATP的镁络合物对该酶的活性也没有影响，尽管游离核苷酸是一种激活剂。这些结果表明，在考虑这些核苷酸的调节作用时，考虑镁络合物的形成很重要。
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