Semenov M A, Bol'bukh T V
Biofizika. 1984 May-Jun;29(3):377-82.
By the method of gravimetry and piezomicrobalance native and denaturated DNA hydration isotherms are obtained. Their adsorptional ability difference observed is interpreted according to IR-data, which show that if sugar-phosphate skeleton hydration levels are similar for these conformations, their nitrous base hydration is strongly different. BET-fitting of isotherms obtained discovered significant water binding energy heterogeneity of native DNA hydration-active sites.
通过重量法和压电力显微镜方法获得了天然和变性DNA的水合等温线。根据红外数据对观察到的它们的吸附能力差异进行了解释,红外数据表明,如果这些构象的糖 - 磷酸骨架水合水平相似,它们的含氮碱基水合则有很大差异。对所获得的等温线进行BET拟合发现天然DNA水合活性位点存在显著的水结合能非均一性。