Lerche D, Bäumler H
Biorheology. 1984;21(3):393-403. doi: 10.3233/bir-1984-21308.
Rouleau formation by back scattered light and flow behaviour by viscometry of heat-treated (48.4, 48.8 and 49.5 degrees C) and normal (37 degrees C) human red blood cells (RBC) were investigated. Washed RBC were treated in PBS and afterwards resuspended in their own plasma. It was found that the time behaviour of the fibrinogen mediated weak RBC-RBC interaction is influenced (decreased) already at an incubating temperature of 48.4 degrees C. Kinetic measurements are more sensitive than a static aggregation characterization. Beside the decreased deformability of the RBC also a heat-altered structure of the RBC-glycocalyx and the aggregating energy of the macromolecules have to be considered.
研究了通过背散射光形成的缗钱状聚集以及通过粘度测定法测定的热处理（48.4、48.8和49.5摄氏度）和正常（37摄氏度）人红细胞（RBC）的流动行为。洗涤后的红细胞在磷酸盐缓冲盐水中处理，然后重悬于自身血浆中。结果发现，在48.4摄氏度的孵育温度下，纤维蛋白原介导的弱红细胞-红细胞相互作用的时间行为就已受到影响（降低）。动力学测量比静态聚集表征更敏感。除了红细胞变形性降低外，还必须考虑红细胞糖萼的热改变结构和大分子的聚集能量。
