Rozenberg A E, Bordiukova I L, Markov Kh M
Biull Eksp Biol Med. 1984 Jul;98(7):121-2.
Three different methods of blood collection from rats are studied and compared, namely decapitation, catheterization of the carotid artery and puncture of the heart left ventricle. The latter method is preferable in studies of platelet aggregation. The method for isolation of washed platelets from rat blood is described in detail. The platelets stored for several hours at 20 degrees C did not lose the ability for aggregating under exposure to low concentrations of ADP. The curves of aggregation of washed and plasma platelets are provided. To study the rate of aggregation, a model is offered based on the approximation of the aggregation curves by the exponential A = A0exp (-alpha t). Such an approach made it possible to treat the data objectively and to reveal the features of platelet functional activity, particularly in arterial hypertension.
研究并比较了三种从大鼠采集血液的不同方法，即断头法、颈动脉插管法和心脏左心室穿刺法。后一种方法在血小板聚集研究中更可取。详细描述了从大鼠血液中分离洗涤血小板的方法。在20℃下储存数小时的血小板在暴露于低浓度ADP时不会丧失聚集能力。给出了洗涤血小板和血浆血小板的聚集曲线。为研究聚集速率，提供了一个基于用指数A = A0exp (-αt)对聚集曲线进行近似的模型。这种方法使得能够客观地处理数据并揭示血小板功能活性的特征，特别是在动脉高血压方面。
