Harding C F, Walters M J, Parsons B
Brain Res. 1984 Jul 23;306(1-2):333-9. doi: 10.1016/0006-8993(84)90383-4.
Using a synthetic, non-metabolizable ligand, R1881 (methyltrienolone), an in vitro binding assay was developed to quantify levels of cytosol androgen receptors in microdissected nuclei from the finch brain. Saturable, high affinity binding in the nanomolar range was demonstrated in the brain areas examined, and receptor levels were unaffected by freezing the tissue samples. The assay was specific for androgen receptors when 10 microM triamcinolone acetonide was added to inhibit the binding of R1881 to glucocorticoid and progestin receptors. Levels of cytosol androgen receptors were quantified in 3 hypothalamic and 3 vocal control nuclei presumed to contain high concentrations of androgen receptors on the basis of previous autoradiography. All nuclei examined showed significant levels of androgen receptors ranging from 5.8 to 35.8 fmol/mg protein. Hypothalamic nuclei had higher concentrations than vocal control nuclei.
使用一种合成的、不可代谢的配体R1881（甲基三烯醇酮），开发了一种体外结合测定法，以定量雀科鸣禽脑微切割细胞核中的胞质雄激素受体水平。在所检查的脑区中证明了在纳摩尔范围内的可饱和、高亲和力结合，并且受体水平不受冷冻组织样本的影响。当加入10微摩尔曲安奈德以抑制R1881与糖皮质激素和孕激素受体的结合时，该测定法对雄激素受体具有特异性。根据先前的放射自显影，在3个下丘脑和3个假定含有高浓度雄激素受体的发声控制核中对胞质雄激素受体水平进行了定量。所有检查的核均显示出显著水平的雄激素受体，范围为5.8至35.8飞摩尔/毫克蛋白质。下丘脑核中的浓度高于发声控制核。
