Gibson B L, Reif-Lehrer L
Brain Res. 1984 Jul;317(1):97-103. doi: 10.1016/0165-3806(84)90144-5.
The onset and developmental pattern of kainate effects were determined in isolated embryonic and posthatching chick neural retinas exposed to KA (0.01-0.24 mM; 1-30 min) in vitro. Damage was first apparent in day 8 embryonic retinas; cells in the inner region of the inner nuclear layer and neuronal processes in the inner plexiform layer were affected. In older embryonic retinas, cells in the inner region of the inner nuclear layer continued to be the most damaged although cells in the ganglion cell layer and the outer region of the inner nuclear layer, and neurites in the outer plexiform layer were also affected. Cells in the outer nuclear layer stained normally in both embryonic and posthatching retinas. Partial reversibility of kainate effects was observed in retinas which had been exposed briefly to kainate and then allowed to recover in kainate-free medium. The results of our in vitro experiments are consistent with those reported for a variety of neonatal and adult retinas treated with kainate in vivo.
在体外将分离的胚胎期和孵化后雏鸡神经视网膜暴露于KA（0.01 - 0.24 mM；1 - 30分钟），以此确定海人酸效应的起始和发展模式。损伤最初在第8天的胚胎视网膜中明显可见；内核层内部区域的细胞以及内网状层中的神经元突起受到影响。在较老的胚胎视网膜中，内核层内部区域的细胞仍然是受损最严重的，尽管神经节细胞层和内核层外部区域的细胞以及外网状层中的神经突也受到了影响。在胚胎期和孵化后视网膜中，外核层的细胞染色正常。在短暂暴露于海人酸然后在无海人酸培养基中恢复的视网膜中，观察到海人酸效应具有部分可逆性。我们的体外实验结果与体内用海人酸处理的各种新生和成年视网膜的报道结果一致。
