Hyndman A G
Invest Ophthalmol Vis Sci. 1984 May;25(5):558-63.
Two different monolayer culture preparations were used to investigate the effects of glutamate and kainate on retinal cell proliferation. Glial monolayer cultures were produced from 8-day-old chick embryo neural retina. Glial monolayers were exposed to glutamate (5 mM) or kainate (0.4 mM) at either 3 or 5 days in vitro. It was found that exposure to glutamate at 3 days in vitro significantly reduced the number of glia present at 6 days, but a day 5 exposure or exposure to kainate did not significantly affect the number of glia. This glutamate-induced decrease in cell number was seen even though thymidine kinase (EC 2.7.75) and thymidylate synthetase (EC 2.1.1-) activities were higher in treated cultures. Neuroblast monolayer cultures were produced from 6-day-old chick embryo neural retina. These cultures also were exposed to glutamate (5 mM) or kainate (0.4 mM) Glutamate, but not kainate treated cultures, showed a decrease in neuroblast survival and proliferation compared with controls. Thus, at the concentrations tested, glutamate appears to be a general retinal toxin, while the survival of immature neurons or glia is not affected by kainate.
使用两种不同的单层培养制剂来研究谷氨酸和红藻氨酸对视网膜细胞增殖的影响。胶质单层培养物由8日龄鸡胚神经视网膜制备。在体外3天或5天时，将胶质单层暴露于谷氨酸（5 mM）或红藻氨酸（0.4 mM）。结果发现，在体外3天时暴露于谷氨酸会显著减少6天时存在的胶质细胞数量，但在第5天暴露或暴露于红藻氨酸对胶质细胞数量没有显著影响。即使在处理过的培养物中胸苷激酶（EC 2.7.75）和胸苷酸合成酶（EC 2.1.1-）的活性较高，仍观察到谷氨酸诱导的细胞数量减少。成神经细胞单层培养物由6日龄鸡胚神经视网膜制备。这些培养物也暴露于谷氨酸（5 mM）或红藻氨酸（0.4 mM）。与对照相比，谷氨酸处理的培养物而非红藻氨酸处理的培养物显示成神经细胞存活和增殖减少。因此，在所测试的浓度下，谷氨酸似乎是一种一般性的视网膜毒素，而红藻氨酸不会影响未成熟神经元或胶质细胞的存活。
