Litchfield W J, Craig A R, Frey W A, Leflar C C, Looney C E, Luddy M A
Clin Chem. 1984 Sep;30(9):1489-93.
Recent innovations in particle design have led to the development of highly sensitive and reproducible immunoassay methods for the Du Pont aca discrete clinical analyzer. Key advances include the synthesis and use of particles less than 1 micron in diameter with high refractive index cores surrounded by thin, chemically reactive shells. The cores are prepared by emulsion polymerization to a well-defined size that depends on the choice of monomer and the requirements for turbidimetric signal. Methods for measuring therapeutic drugs (e.g., theophylline) involve particles with polystyrene cores; other methods require cores with higher refractive indices such as polyvinylnaphthalene. The shells are critical for overall method performance because they bind covalently the immunochemicals of interest. Polyglycidyl methacrylate shells have been used effectively to attach antigens and haptens to the particle surface.
颗粒设计方面的最新创新成果，促成了用于杜邦aca离散式临床分析仪的高灵敏度且可重复的免疫分析方法的开发。关键进展包括合成并使用直径小于1微米的颗粒，其具有高折射率的内核，并被薄的、具有化学反应活性的外壳所包围。内核通过乳液聚合制备成尺寸明确的颗粒，其大小取决于单体的选择以及比浊法信号的要求。测量治疗药物（如茶碱）的方法涉及具有聚苯乙烯内核的颗粒；其他方法则需要具有更高折射率的内核，如聚乙烯萘。外壳对于整体方法性能至关重要，因为它们将感兴趣的免疫化学物质共价结合。聚甲基丙烯酸缩水甘油酯外壳已被有效地用于将抗原和半抗原附着到颗粒表面。
