Darkow R, Groth T, Albrecht W, Lützow K, Paul D
GKSS Research Center, Institute of Chemistry, Teltow, Germany.
Biomaterials. 1999 Jul;20(14):1277-83. doi: 10.1016/s0142-9612(99)00022-8.
Nanoparticles consisting of a polystyrene core and a polyglycidyl methacrylate shell were prepared by a two-step emulsion polymerization. The size and surface properties of the particles were characterized by scanning electron microscopy, dynamic light scattering and polyelectrolyte titration techniques. Particles were found to be monodisperse with a mean diameter of about 85 nm. Parent particles were modified with a number of different ligands including diamines of increasing chain length, amino acids and corresponding amines and higher molecular weight ligands like polymyxin B. The modified particles were tested for their endotoxin (ET) binding capacity in water and physiological sodium chloride solution with the Limulus amebocyte lysate (LAL) assay. It was found that the ET binding properties of the different ligands depend both on the ability of the ligand to form Coulomb- and van der Waals-interactions with the ET molecule influenced by the nature of the suspension medium. Therefore, the choice of ligands for particle modification has to consider minutely the conditions under which ET has to be removed, e.g. removal from pure water, dialysis fluids, plasma or blood.
通过两步乳液聚合制备了由聚苯乙烯核和聚甲基丙烯酸缩水甘油酯壳组成的纳米颗粒。通过扫描电子显微镜、动态光散射和聚电解质滴定技术对颗粒的尺寸和表面性质进行了表征。发现颗粒呈单分散，平均直径约为85nm。用多种不同的配体修饰母体颗粒，包括链长增加的二胺、氨基酸和相应的胺以及更高分子量的配体如多粘菌素B。用鲎试剂法在水和生理氯化钠溶液中测试修饰颗粒的内毒素（ET）结合能力。发现不同配体的ET结合特性既取决于配体与ET分子形成库仑相互作用和范德华相互作用的能力，又受悬浮介质性质的影响。因此，用于颗粒修饰的配体选择必须仔细考虑去除ET的条件，例如从纯水、透析液、血浆或血液中去除。
