Jones R A
Biochem Genet. 1978 Feb;16(1-2):27-38. doi: 10.1007/BF00484382.
Zein accumulation patterns during mutant and normal maize endosperm development were determined. Accompanying an increase in the number of floury-2 alleles present in the endosperm was a well-defined stepwise depression in zein accumulation. Analysis of the zein accumulated in endosperms containing zero, one, two, and three doses of the floury-2 allele by sodium dodecylsulfate--polyacrylamide gel electrophoresis revealed a proportionate reduction in the two major zein components, Z1 and Z2. In contrast, the relative proportions of the minor zein bands were altered. Membrane-bound polysomes isolated from kernels of floury-2 and normal maize were predominantly large size classes. The presence of increasing numbers of the floury-2 allele in the endosperm decreased recovery of membrane-bound polysomal material in a stepwise fashion. However, major alterations in polysome size-class distributions were not observed. The reduction in membrane-bound polysome material correlated linearly with reductions in in vitro zein synthesis and in vivo zein accumulation.
测定了突变型和正常型玉米胚乳发育过程中玉米醇溶蛋白的积累模式。随着胚乳中粉质-2等位基因数量的增加，玉米醇溶蛋白的积累出现了明确的逐步下降。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析含有零剂量、一剂量、二剂量和三剂量粉质-2等位基因的胚乳中积累的玉米醇溶蛋白，发现两种主要的玉米醇溶蛋白成分Z1和Z2成比例减少。相比之下，次要玉米醇溶蛋白条带的相对比例发生了变化。从粉质-2玉米和正常玉米的籽粒中分离出的膜结合多核糖体主要是大尺寸类别。胚乳中粉质-2等位基因数量的增加导致膜结合多核糖体物质的回收率逐步下降。然而，未观察到多核糖体大小类别分布的主要变化。膜结合多核糖体物质的减少与体外玉米醇溶蛋白合成和体内玉米醇溶蛋白积累的减少呈线性相关。
