Lee K H, Jones R A, Dalby A, Tsai C Y
Biochem Genet. 1976 Aug;14(7-8):641-50. doi: 10.1007/BF00485842.
Sodium dodecylsulfate-polyacrylamide gel electrophoresis reveals that zein prepared from normal maize inbred (Zea mays L.) contains six separable components. Z1 and Z2 are the predominant species, with molecular weights of 21,800 and 19,000 daltons. Amino acid analysis of these two components shows that both are rich in glutamic acid, leucine, and proline, but low in lysine. Of the four minor bands, Z3, Z4, Z5, and Z6, the latter two exist only in trace amounts. A mutation at the opaque-2 locus severely suppresses the synthesis of Z1. The nonallelic mutant, opaque-7, strongly suppresses the synthesis of Z3 and Z4, while slightly reducing Z2. On the other hand, the floury-2 mutant appears to reduce the synthesis of these six proteins in the same relative proportion. In the double mutant combinations, opaque-2 apparently is epistatic to opaque-7 and floury-2 in the synthesis of zein components. The glutelin fraction shows a more complex banding pattern; however, qualitative differences are not apparent among the mutant lines examined.
十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示，由普通玉米自交系（Zea mays L.）制备的玉米醇溶蛋白含有六个可分离的组分。Z1和Z2是主要组分，分子量分别为21,800和19,000道尔顿。对这两个组分的氨基酸分析表明，它们都富含谷氨酸、亮氨酸和脯氨酸，但赖氨酸含量较低。在四个次要条带Z3、Z4、Z5和Z6中，后两个仅以痕量存在。opaque-2位点的突变严重抑制Z1的合成。非等位突变体opaque-7强烈抑制Z3和Z4的合成，同时略微降低Z2。另一方面，粉质-2突变体似乎以相同的相对比例降低这六种蛋白质的合成。在双突变体组合中，在玉米醇溶蛋白组分的合成中，opaque-2显然对opaque-7和粉质-2是上位性的。谷蛋白组分显示出更复杂的条带模式；然而，在所检查的突变系之间没有明显的定性差异。
