牛角膜基质硫酸角质素蛋白聚糖的特性分析。
Characterization of the keratan sulphate proteoglycans from bovine corneal stroma.
作者信息
Axelsson I, Heinegård D
出版信息
Biochem J. 1978 Mar 1;169(3):517-30. doi: 10.1042/bj1690517.
Abstract
The keratan sulphate proteoglycans that can be prepared from bovine corneal stroma [Axelsson & Heinegård (1975) Biochem. J. 145, 491-500] were characterized by gel chromatography, gel electrophoresis and analytical ultracentrifugation in associative (0.6 M-NaCl) and dissociative (6M-guanidinum chloride) solvents. The proteoglycans aggreagated at low salt concentrations and pH. The weight-average molecular weight of the monomer proteoglycans was established. Keratan sulphate peptides and oligosaccharide peptides were isolated after proteolysis. Their composition indicated that both are linked to protein via asparagine residues. A tentative model for corneal keratan sulphate proteoglycans is suggested.
摘要
可从牛角膜基质制备的硫酸角质素蛋白聚糖[阿克塞尔松和海内加德（1975年），《生物化学杂志》145卷，491 - 500页]，通过在缔合性（0.6M氯化钠）和解离性（6M氯化胍）溶剂中的凝胶色谱法、凝胶电泳法和分析超速离心法进行了表征。这些蛋白聚糖在低盐浓度和pH条件下会聚集。确定了单体蛋白聚糖的重均分子量。蛋白水解后分离出了硫酸角质素肽和寡糖肽。它们的组成表明两者均通过天冬酰胺残基与蛋白质相连。提出了角膜硫酸角质素蛋白聚糖的初步模型。
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