Axelsson I, Heinegård D
Biochem J. 1975 Mar;145(3):491-500. doi: 10.1042/bj1450491.
Proteoglycans were extracted from bovine corneal stroma with 4M-guanidinum chloride, purified by DEAE-dellulose chromatography (Antonopoulos et al., 1974) and fractionated by precipitation with ethanol into three fractions of approximately equal weight. One of these fractions consisted of a proteoglycan that contained keratan sulphate as the only glycosaminoglycan. In the othertwo fractions proteoglycans that contained chondroitin sulphate, dermatan sulphate and keratan sulphate were present. Proteoglycans which had a more than tenfold excess of galactosaminoglycans over keratan sulphate could be obtianed by further subfractionation. The gel-chromatographic patterns of the glucosaminoglycans before and after digestion with chondroitinase AC differed for the fractions. The individual chondroitin sulphate chains seemed to be larger in cornea than in cartilage. Oligosaccharides, possibly covalently linked to the protein core of the proteoglycans, could be isolated from all fractions. The corneal proteoglycans were shown to have higher protein contents and to be of smaller molecular size than cartilage proteoglycans.
用4M-氯化胍从牛角膜基质中提取蛋白聚糖，通过DEAE-纤维素色谱法纯化（Antonopoulos等人，1974年），并用乙醇沉淀法将其分成三个重量大致相等的组分。其中一个组分由一种蛋白聚糖组成，该蛋白聚糖仅含有硫酸角质素作为唯一的糖胺聚糖。在另外两个组分中，存在含有硫酸软骨素、硫酸皮肤素和硫酸角质素的蛋白聚糖。通过进一步细分可以获得半乳糖胺聚糖相对于硫酸角质素过量十倍以上的蛋白聚糖。各组分经软骨素酶AC消化前后的糖胺聚糖凝胶色谱图谱有所不同。角膜中单个硫酸软骨素链似乎比软骨中的更大。可能与蛋白聚糖的蛋白核心共价连接的寡糖可以从所有组分中分离出来。结果表明，角膜蛋白聚糖的蛋白质含量更高，分子大小比软骨蛋白聚糖更小。
