水貂与人血清淀粉样蛋白A（SAA）的比较研究。

A comparative study of serum amyloid a protein (SAA) from mink and man.

作者信息

Marhaug G, Børresen A L, Husby G, Nordstoga K

出版信息

Comp Biochem Physiol B. 1984;78(2):401-6. doi: 10.1016/0305-0491(84)90049-x.

Abstract

Serum amyloid A protein (SAA) was isolated from mink and human serum by ultra-centrifugation and gel filtration and characterized by two-dimensional gel electrophoresis and Western blotting followed by autoradiography. SAA was found in similar quantities in the high density lipoprotein (HDL) fraction of serum from a patient suffering from systemic juvenile rheumatoid arthritis (JRA) and mink stimulated with lipopolysaccharide (LPS). Only very small quantities were present in normal human controls and not detectable in normal mink. Striking similarities were found in molecular weight, isoelectric point and degree of heterogeneity for human and mink SAA, while immunologic cross-reactivity between the two species was not found. In contrast to human HDL, mink HDL was found not to contain apoA-II and only minute amounts of apoC proteins.

摘要

通过超速离心和凝胶过滤从水貂和人血清中分离出血清淀粉样蛋白A（SAA），并通过二维凝胶电泳和蛋白质印迹法随后进行放射自显影对其进行表征。在一名患有全身性幼年类风湿性关节炎（JRA）的患者的血清高密度脂蛋白（HDL）组分和用脂多糖（LPS）刺激的水貂中发现了数量相似的SAA。正常人对照组中仅存在极少量，正常水貂中未检测到。人和水貂的SAA在分子量、等电点和异质性程度方面存在显著相似性，而未发现两种物种之间的免疫交叉反应性。与人类HDL相反，发现水貂HDL不含载脂蛋白A-II，仅含有微量的载脂蛋白C蛋白。