Medeiros L O, Nürmberger R, Medeiros L F
Comp Biochem Physiol B. 1984;78(4):869-71. doi: 10.1016/0305-0491(84)90201-3.
Erythrocytes from thoroughbred horses were submitted to total (80-90%) and partial (25-40%) oxidation of hemoglobin by sodium nitrite. The ability of these cells to reduce methemoglobin to hemoglobin in the presence of either glucose, glucose plus methylene blue or lactate was investigated. The results were compared with those ones obtained for human erythrocytes. Under total oxidation: the horse erythrocytes need longer incubation time with glucose or glucose plus methylene blue than human erythrocytes for reducing the methemoglobin; methylene blue did not enhance methemoglobin reduction in the equine erythrocytes, as occurred in human erythrocytes; for horses, lactate was a more efficient substrate in promoting methemoglobin reduction. The reduction of methemoglobin by equine erythrocytes under partial oxidation was very quick in any of the incubation media. The results can explain the incongruity between the previously reported inability of equine erythrocytes to reduce methemoglobin and the lack of methemoglobinemias in equine veterinary practice.
将纯种马的红细胞用亚硝酸钠进行血红蛋白的完全氧化（80 - 90%）和部分氧化（25 - 40%）。研究了这些细胞在葡萄糖、葡萄糖加亚甲蓝或乳酸存在的情况下将高铁血红蛋白还原为血红蛋白的能力。将结果与人类红细胞的结果进行比较。在完全氧化情况下：马红细胞与葡萄糖或葡萄糖加亚甲蓝孵育以还原高铁血红蛋白所需的时间比人类红细胞长；亚甲蓝不像在人类红细胞中那样能增强马红细胞中高铁血红蛋白的还原；对于马来说，乳酸是促进高铁血红蛋白还原更有效的底物。在部分氧化情况下，马红细胞在任何孵育介质中还原高铁血红蛋白都非常迅速。这些结果可以解释先前报道的马红细胞无法还原高铁血红蛋白与马兽医实践中缺乏高铁血红蛋白血症之间的不一致性。
