Wagh U V, Chiplonkar J M, Tyagi N S, Dighe R P
Cytometry. 1984 Jul;5(4):403-7. doi: 10.1002/cyto.990050418.
Results of studies on characterization of mosquito cell lines are described; these include chromosome analysis, image cytometric, and flow cytometric estimation of DNA, tumorogenicity and angiogenicity, concanavalin-A-induced agglutination, and proton spin-lattice relaxation time (T1). The established mosquito cell lines are not diploid lines, although their stem-lines are diploid. Feulgen cytometry and flow cytometry reveal the inherent heterogeneity in DNA contents and support the observations on chromosome frequency distribution. Two cell lines exhibit angiogenic property and cell infiltration in chorio-allantoic membrane of chick embryos; however, none of the cell lines tested by inoculating hamsters, mice, or adult mosquitoes exhibit tumorogenicity. The agglutinability patterns of these cells in response to concanavalin-A treatment reveal the coexistence of agglutinable and unagglutinable cells. The mosquito cells differ from mammalian and chick cells in respect of their proton spin-lattice relaxation time.
本文描述了蚊细胞系特性研究的结果；这些研究包括染色体分析、图像细胞术和流式细胞术对DNA的估计、致瘤性和血管生成性、刀豆球蛋白A诱导的凝集以及质子自旋-晶格弛豫时间（T1）。已建立的蚊细胞系不是二倍体细胞系，尽管其干细胞系是二倍体。福尔根细胞术和流式细胞术揭示了DNA含量的内在异质性，并支持了关于染色体频率分布的观察结果。有两个细胞系在鸡胚绒毛尿囊膜中表现出血管生成特性和细胞浸润；然而，通过接种仓鼠、小鼠或成年蚊子测试的所有细胞系均未表现出致瘤性。这些细胞对刀豆球蛋白A处理的凝集模式揭示了可凝集细胞和不可凝集细胞的共存。蚊细胞在质子自旋-晶格弛豫时间方面与哺乳动物细胞和鸡细胞不同。
